摘要
以ZORBAX Eclipse DB-C18为分析柱,乙腈-甲醇（体积比95∶5）作流动相,建立了饲料中角黄素的反相高效液相色谱分析方法。对饲料样品进行3个添加水平的回收实验分析,回收率为96%-112%,精密度（RSD）为1.90%-3.53%,线性范围为1.0-20.0 mg/L,检出限为0.28 mg/kg。
Canthaxanthin in feed stuff was separated on an ZORBAX Eclipse DB-C18 analytical column（4.6 mm×150 mm,5 μm） at 25 ℃ with acetonitrile and methanol（95 ∶5,by volume）as mobile phase at 1.0 mL·min^-1 and then detected by DAD at 470 nm.The average recoveries were range of 96%-112%with RSD of 1.90%-3.53%.The linear range ranged from 1.0 to 20.0 mg/L.The detection limit was 0.28 mg/kg.This method could be used to determine the content of canthaxanthin in feed stuff.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第7期785-787,共3页
Journal of Instrumental Analysis
