摘要
目的探讨肺癌肿瘤阻抑基因-1(TSLC1)、上皮型钙黏蛋白(E-cad)在宫颈鳞癌组织芯片中的表达及其临床意义,并分析两者的相关性。方法应用免疫组化法和图像分析技术分别对29例宫颈鳞癌组织、20例CINs、10例正常宫颈组织TSLC1、E-cad的含量进行研究。结果TSLC1、E-cad在正常宫颈及CINⅠ级组织中,多呈强阳性表达,CINⅡ级以上及宫颈鳞癌组织表达呈递减趋势,差异有显著性(P<0.05)。宫颈鳞癌组织中TSLC1蛋白表达失活与淋巴转移有关,差异有显著性(t=4.558,P=0.000);与组织病理分级无关(P>0.05)。E-cad蛋白表达失活与组织病理学分级和淋巴转移有关,差异有显著性(P<0.05)。TSLC1蛋白、E-cad蛋白表达呈正相关(r=0.678,P=0.000)。结论组织芯片是大规模平行检测多基因蛋白表达的一种方法;TSLC1、E-cad蛋白表达失活使其丧失了抑癌作用,是促使正常宫颈上皮细胞癌变的一个重要因素。
[Objective] To detect the expression of TSLC1 and E-cad in cervical cancer by tissue microarray and to evaluate their clinical significance and correlation between the two genes. [Methods] 59 specimens of tissues, including squamous carcinoma of the cervix, CINs and normal tissues underwent microarray examination and immuno- histochenmistry to detect the expression of TSLC1, E-cad. The intensity of TSLC1 and E-cad protein were investigated by analyzing with imaging technique. [Results] The result showed that the expression of strong positive cell in normal cervical tissue and CIN Ⅰ were significantly higher than those of CIN Ⅱ, CIN Ⅲ and squamous carcinoma of the cervix. There was no association between TSLC1 expression and histological type. But the expression of TSLC1 was significantly correlated with lymphatic metastasis. Inactivation of E-cad gene expression in cervical cancer was significantly related to do with lymphatic metastasis and histological type. The expression of TSLC1 and E-cad in cervical tissue were positive correlated （r=0.678, P=0.000）. [Conclusion] Tissue microarray technique is effective method to detect multiple gene protein expression. Inactivation of TSLC1, E-cad gene expression is an important factor in the uterine cervical carcinogenesis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第13期1801-1803,1809,共4页
China Journal of Modern Medicine
