摘要
目的:研究三棱、莪术抑制海绵内新生肉芽组织血管新生的作用及其可能机制。方法:复制大鼠皮下海绵移植模型,连续灌胃给药2周,观察三棱、莪术对海绵内新生肉芽组织生长及其新生血管的影响;通过免疫组化法进行内皮细胞特异性Ⅷ因子、血管内皮细胞生长因子(VEGF)染色;逆转录-聚合酶链反应法检测VEGFmRNA的表达。结果:三棱、莪术高剂量组大鼠海绵内新生肉芽组织侵入生长、血管数量、VEGF蛋白及VEGFmRNA的表达量均较模型组显著减少(P<0.01或P<0.05)。结论:三棱、莪术可抑制海绵内新生肉芽组织的血管新生,其机制可能与抑制新生血管表达VEGF有关。
OBJECTIVE ： To explore the inhibitory effects and mechanism of Rhizoma Sparganii and Rhizoma Curcumae on the angiogenesis of new granulation tissue in sponge. METHODS： The experimental endometriosis in female rats was established with sponge implantation. The effects of Rhizoma Sparganii and Rhizoma Curcumae on the angiogenesis of new granulation tissue in sponge were observed after intragastric medication for 2 consecutive weeks. The staining of endothelial cell specific Ⅷ factor and vascular endothelial cell growth factor（VEGF） was carried out by immunohistochemistry method. The expression of VEGF mRNA was detected by RT- PCR assay. RESULTS： In rats receiving high dosage of Rhizoma Sparganii and Rhizoma Curcumae（SC）,the area of new granulation tissue in sponge,the number of blood vessels, the expression of VE- GF and VEGF mRNA were all significantly lower than in model group（P 〈 0.01 or P 〈0.05） . CONCLUSION： SC could inhibit the angiogenesis of new granulation tissue in sponge, and its mechanism might be associated with the inhibition of the expression of VEGF in the new vessel.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第21期1610-1612,共3页
China Pharmacy
关键词
三棱
莪术
血管新生
血管内皮细胞生长因子
Rhizoma Sparganii
Rhizoma Curcumae
Angiogenesis
Vascular endothelial cell growth factor（VEGF）
