摘要
为增加纤维素的酶解反应速率，通过降解羧甲基纤维素钠、微晶纤维素、纤维素粉Sigmacell type50，研究梅林青霉（Penicillium Melinii Thom）Z18菌株纤维素酶的特性和作用条件．结果表明：纤维素底物浓度影响酶降解效率，纤维素浓度高于5％时，产生明显底物抑制，50～70℃和pH5．5是纤维素酶解最佳温度和酸碱度，酶解时间的延长有利于降解，但反应超过2h，还原糖增加缓慢，K^＋和Mn^2＋能够增加纤维素酶降解羧甲基纤维素钠和纤维素粉Sigmacell type50效率，Fe^2＋和Cu^2＋对于所有纤维素酶解表现抑制，较低结晶度的羧甲基纤维素钠降解速率高于较高结晶度的纤维素粉，纤维素酶水解作用条件的优化和纤维素结晶度的降低能够提高纤维素酶的水解反应速率．
For improving the hydrolysis rate of cellulose, the properties and acting conditions of Penicillium Melinii Thorn Z18 strain cellulase were investigated through degradation of carboxymethyl- cellulose sodium, microcrystalline cellulose and Sigmacell type50. The results showed that the activity of cellulase was evidently inhibited when the substrate concentration of cellulose was more than 5%, the optimum temperature and acidity of cellulase to degrade model cellulose were 50 -70 ℃ and pH5. 5, the release of reductive sugar becomes slow with reaction time more than 2 h. K^＋ and Mn^2＋ ion could increase the reductive sugar release of carboxymethyl- cellulose and Sigmacell type50, whereas Fe^2＋ and Cu^2＋ ion showed inhibition to hydrolysis of all model cellulose, the degradation efficiency of carboxymethyl - cellulose was higher than that of microcrystalline cellulose and Sigmacell type50. Degradation efficiency of cellulose could be increased through optimizing hydrolysis conditions of cellulase and decreasing the crystalline index of cellulose.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2008年第6期919-922,共4页
Journal of Harbin Institute of Technology
基金
黑龙江省科技攻关项目(GC04C209)
关键词
纤维素
梅林青霉
纤维素酶
底物抑制
cellulose
Penicillium melinii
cellulase
substrate inhibition
