摘要
为探讨氯化甲基汞对雌性动物生殖系统的影响，采用氯化甲基汞给雌性昆明种小鼠急性染毒，剂量为１／２００ＬＤ５０、１／２０ＬＤ５０和１／２ＬＤ５０。染毒后采用ＦＡＣ－Ｓｃａｎ流式细胞仪技术分析了氯化甲基汞对卵巢细胞周期的影响。结果表明：１／２０ＬＤ５０组和１／２ＬＤ５０组中处于Ｇ０／Ｇ１期时相的细胞百分数高于对照组（Ｐ＜０．０５），而１／２０ＬＤ５０组和１／２ＬＤ５０组的Ｓ期时相的细胞的百分数明显低于对照组（Ｐ＜０．０５），其它无明显改变。研究表明，甲基汞可以造成Ｇ１期细胞增加，出现明显的Ｇ１阻滞，ＤＮＡ合成受抑制。
The ovary cell cycles were analyzed using flow cytometry(FCM) after female Kunming strain mice were acutely exposed to methlmercury with dose of 1/200 LD50,1/20 LD50and 1/2 LD50 to explore the effect of methylmercury cholride on female animal productive system.The effect showed that the ovary cells in G0/G1 phase increased significantly (P<005) compared with those in control groups,and in S phase decreased significantly in 1/20 LD50and 1/2 LD50 groups,other obvious changes were not observed.It indicated that methylmercury could cause the increase of the cells and obvious block in G1 phrase,inhibit DNA synthesis and delay cell mitosis.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1997年第6期252-253,共2页
Journal of Environment and Health
基金
国家自然科学基金
关键词
卵巢
细胞周期
氯化甲基汞
Ovary cell cycle Methylmercury chloride Flow cytometry(FCM)
