摘要
从某焦化废水生物处理装置的活性污泥中分离筛选出一株菲的降解优势菌株,经形态学观察和ITS序列分析,对菌种进行了鉴定.考察了菌株对水中多环芳烃菲的降解特性.结果表明,该菌可在以菲为唯一碳源的培养基中生长.在蔡司显微镜下观察,菌株的菌丝呈树枝状,颜色呈白色.通过ITS序列分析,初步鉴定所得菌株为茄镰孢菌（Fusarium so-lani）或该菌的一个株系.所得茄镰孢菌为好氧真菌.投菌量、初始菲浓度和H2O2浓度对菲的降解影响较大.菲的最适宜降解条件为：投菌量8%-10%,pH值为6.0-8.0,H2O2用量为70 mg/L.在此适宜条件下,对于初始浓度为40 mg/L的菲溶液,在转速120 r/min、水温为30℃的摇床中培养5 d后,菲的去除率可达86.7%.
A fungous strain degrading phenanthrene is isolated and screened from the activated sludge fetched from the biological treatment unit of coking wastewater. The strain is initially identified with the results of morphological observation and ITS sequence analysis. The biodegradation characteristics of phenanthrene by the strain are investigated. Results show that the strain can grow on medium containing phenanthrene as sole carbon source. The strain whose hypha appears branch-shape and white is initially identified as Fusarium solani with the results. The strain Fusarium solani is aerophile fungus. The added fungus Fusarium solani concentration, the initial concentration of phenanthrene and the concentration of H2O2 have obviously effects on the degradation efficiency. The optimum biodegradation conditions of phenanthrene by strain Fusarium solani are as follows： the fungus concentration is 8%- 10% , pH is 6.0 -8.0 and H2O2 concentration is 70 mg/L. Under these conditions, the degradation efficiency of phenanthrene in triangle flask in shaking bed with the rotation speed 120 r/min and water temperature 30℃ can be reached to more than 86.7% for the phenanthrene solution with the initial concentration of 40 mg/L.
出处
《江苏科技大学学报（自然科学版）》
CAS
北大核心
2008年第3期72-76,共5页
Journal of Jiangsu University of Science and Technology:Natural Science Edition
基金
江苏省自然科学基金资助项目(BK2005057)
江苏省高校自然科学基金资助项目(03KJD610013)
关键词
茄镰孢菌
分离筛选
菲
生物降解
Fusarium solani
isolating and screening
phenanthrene
biodegradation
