摘要
高节竹笋加工废料笋蔸蛋白质含量高达20.6%,而木素含量仅为2.7%;笋壳的蛋白质含量为12.3%,纤维素、戊聚糖和木素含量分别为32.8%,25.0%和13.2%。笋壳经稀硫酸预处理后,用里氏木霉作产酶菌,5d后可得到滤纸酶活为1.9IU·mL-1的酶液,产酶渣蛋白质含量提高1倍,达25.4%。用自产酶液酶解预处理后的废料,酶用量为12IU·g-1绝干原料时,笋壳和笋蔸的酶解得率分别为80.1%和82.0%。酶水解液和预处理液可培养酵母。
Canned bamboo shoot residues of Phyllostachys prominens consists of bamboo shoot sheath(culm leaf)and bamboo shoot(culm) Bamboo shoot(culm)was rich in protein(20 6%)and poor in lignin(2 7%);bamboo shoot sheath(culm leaf)contains protein(12 3%),cellulose(32 8%),pentosan(25 0%)and lignin(13 9%) Dilute H 2SO 4 prehydrolysed bamboo shoot sheath was used as carbon source in cellulase production by Trichoderma reesei Rut C30 After 5 days,obtained enzyme solution had a FP active of 1 9 IU·mL -1 and cellulase production residue was more proteinous(25 4% protein)than shoot sheath Dilute H 2SO 4 prehydrolused bamboo shoot residues used as substrate,enzyme substrate ratio 12 IU·g -1 dry residues The yield of enzymatic hydrolysis could reach 80 1%(culm leaf)and 82 0%(culm) The sugar solution of prehydrolysis and enzymatic hydrolysis could be used to produce yeast The residues of cellulase production and enzymatic hydrolysis increased in feed value
出处
《浙江林学院学报》
CSCD
1997年第3期262-266,共5页
Journal of Zhejiang Forestry College
关键词
笋加工废料
高节竹
纤维素酶
饲料
canned bamboo shoot residues
Phyllostachys prominens
cellulase
feeds