摘要
将人外周血淋巴细胞（ＰＢＬ）和腹腔淋巴结淋巴细胞（ＰＬＮＬ）移植入严重联合免疫缺陷疾病（ＳｅｖｅｒｅＣｏｍｂｉｎｅｄＩｍｍｕｎｏｄｅｆｉｃｉｅｎｔＤｉｓｅａｓｅ，ＳＣＩＤ）小鼠腹腔，建立人化ＳＣＩＤ小鼠（即ＳＣＩＤ－ｈＰＢＬ，ＳＣＩＤ－ｈＰＬＮＬ）。两个月后，两种人化小鼠体内淋巴器官都可以检测到人Ｔ、Ｂ淋巴细胞分布；小鼠血清人源性ＩｇＧ和抗ＥＢ病毒壳抗原ＩｇＧ抗体水平（ＩｇＧ／ＶＣＡ）比较显示，用Ｂ９５８死细胞作为ＶＣＡ来源所诱导的实验组１０只小鼠，ＩｇＧ／ＶＣＡ阳性率为７０％（７／１０），对照组为１７％（２／１２）。１４只ＳＣＩＤ－ｈＰＬＮＬ小鼠血清内人ＩｇＧ／ＶＣＡ的几何平均滴度（ＧＭＴ）和血清ＩｇＧ浓度在１∶１０８和９６．２±５６．４μｇ／Ｌ；在另８只ＳＣＩＤ－ｈＰＢＬ中为１∶７．９和１３．８４±６．０μｇ／Ｌ。实验提示，ＳＣＩＤ－ｈＰＬＮＬ小鼠较ＳＣＩＤ－ｈＰＢＬ小鼠更适合于人源性特异ＩｇＧ的诱导。
SCID hPBL and SCID hPLNL mice models have been established by intraperitoneal injection of lymphocytes isolated from normal human peripheral blood or peritoneal lymph nodes into severe combined immunodeficient disease (SCID) mice.The mice were then immunized by EB VCA positive B958 cells.Two months after reconstitution of Hu SCID mice,the presence of human B cells(CD20 +) and T cells(CD3 +) were shown in the spleen,thymus and peritoneal lymph nodes in both Hu SCID mice with immunoperoxidase staining technique.The sera from the SCID mice were then tested for the level of human IgG and induction of human IgG to EB VCA (IgG/VCA).The results revealed that 70%(7/10) of sera had positive human IgG/VCA in the immunized mice,in comparison to 17%(2/12) in the control group.The GMT of human IgG/VCA and the level of human IgG in the sera were 1∶108 and 96 2±56 4 μg/L in 14 SCID hPLNL mice and 1∶7 9 and 13 84±6 0μg/L in 8 SCID hPBL mice,respectively.The results suggest that SCID hPLNL mice may be more effective for induction of human IgG to some specific immunogen than the SCID hPBL mice.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第5期321-323,共3页
Chinese Journal of Microbiology and Immunology
基金
广东省科委重大项目基金
关键词
免疫应答性
免疫缺陷
淋巴细胞
IGG
Immune response Immuno deficient disese Lymphocytes \ IgG
