摘要
为探讨福氏痢疾杆菌（Ｆ２ａ）感染后胃肠相关淋巴组织（ＧＡＬＴ）中的免疫应答状况，对口服Ｆ２ａ免疫后，ＢＡＬＢ／ｃ小鼠ＧＡＬＴ效应部位上皮内（ＩＥ）和固有膜内（ＬＰ）淋巴细胞表型进行了ＦＡＣＳ分析，并与脾、肠系膜淋巴结（ＭＬＮ）和Ｐｅｙｅｒ结（ＰＰ）中的变化进行了比较。发现脾Ｌ３Ｔ４＋和Ｌｙｔ２＋细胞亚群的变化相对很小；ＭＬＮ中的变化较大，免疫后第４天Ｌ３Ｔ４＋和Ｌｙｔ２＋细胞都明显增加；ＰＰ中的变化明显，于末次免疫后第１天，Ｌｙｔ２＋细胞明显增加；上皮内Ｌ３Ｔ４＋细胞在前１０天内相对增加；固有膜内Ｌ３Ｔ４＋细胞在前７天不断增加，但因Ｌｙｔ２＋在第１天大量增加，Ｌ３Ｔ４＋／Ｌｙｔ２＋在第１天明显变小。这些结果表明：Ｆ２ａ多次口服免疫后，Ｔ细胞免疫应答主要发生于ＧＡＬＴ中；ＰＰ中的变化可能早于ＭＬＮ；ＩＥ可能直接对通过上皮的抗原进行免疫应答；除Ｌ３Ｔ４＋Ｔ细胞外，Ｌｙｔ２＋Ｔ细胞可能在粘膜免疫应答的调节中起着独特而十分重要的作用。
L3T4 + and Lyt2 + T cell subsets in the gut associated lymphoid tissues (GALT) of BALB/c mice immunized orally with Shigella flexneri 2a 4 times were analysed with cytometry to gain an insight into the cell mediated immunity of F2a infection.It was found that both L3T4 + and Lyt2 + T cells changed significantly in the GALT rather than in the spleen.Most of the changes occurred within the first 10 days.There was a relative rise of CD8 + T cells in PP and LP,while a rise of CD4 + T cells in MLN and IE occurred.These results indicate CD8 + T cells,other than CD4 + T cells,might play a unique and very important part in the cell mediated immune responses in the GALT of F2a orally immunized mice.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第4期247-250,共4页
Chinese Journal of Microbiology and Immunology
关键词
L3T4^+
淋巴组织
痢疾杆菌
淋巴细胞亚群
WT5"
6BZ]L3T4 +Lyt2 +Lymphoid tissues Shigella flexneri \ \ Lymphocyte subsets
