摘要
用３Ｈ－ＴｄＲ掺入法，观察了经抗γδ＋Ｔ细胞的单抗处理和Ｈ３７Ｒｖ免疫的Ｃ５７ＢＬ／６小鼠淋巴细胞体外对ＭＰＴ５９刺激的增殖反应。结果显示，去除γδ＋Ｔ细胞不能阻止该淋巴细胞的增殖。ＦＡＣＳｃａｎ分析表明，被ＭＰＴ５９刺激增殖的Ｔ细胞３１％为Ｖβ１１＋Ｔ细胞，其明显高于非刺激组和ＤＮＰ－ＫＬＨ刺激组。ＭＰＴ５９以Ｖβ１１为主要方式刺激Ｔ细胞增殖，提示它可能是结核杆菌分泌的一种超抗原。
With the method of 3 H TdR incorporation,the proliferative response of lymphocytes from the mice treated with anti γδ +T cell mAb and immunized with H37Rv strain to MPT59 were observed in vitro .The results showed deletion of γδ +T cell could not block the proliferation of the lymphocytes.With analysis of flow cytometer,it was found that 31% of the proliferative T cells stimulated by MPT59 was Vβ11 +T cells,which was obviously higher than those of the unstimulated group and DNP KLH stimulated group.MPT59 stimulating the T cell proliferation in a Vβ 11 specific manner suggests that it may be a superantigen secreted by M.tuberculosis.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第4期244-246,共3页
Chinese Journal of Microbiology and Immunology
关键词
结核杆菌
超抗原
T淋巴细胞
MPT59\ \ M.tuberculosis \ \ Superantigen T lymphocytes
