摘要
为了探讨紫外线诱发和加重皮肤红斑狼疮的机理，利用体外培养的人角朊细胞，通过间接免疫荧光法检测ＵＶＢ照射对角朊细胞表达Ｒｏ／ＳＳＡ抗原的影响。结果表明：角朊细胞在接受不同剂量ＵＶＢ（５０ｍＪ／ｃｍ２，１００ｍＪ／ｃｍ２，２００ｍＪ／ｃｍ２）照射后细胞膜出现Ｒｏ／ＳＳＡ抗原表达，并与ＵＶＢ剂量有相关性，２００ｍＪ／ｃｍ２的ＵＶＢ照射可使１０．７％的角朊细胞膜表达Ｒｏ／ＳＳＡ抗原。角朊细胞若先与１０－５ｍｏｌ／Ｌ１７－β－雌二醇孵育４８小时再接受ＵＶＢ照射（２００ｍＪ／ｃｍ２），结果较单纯接受相同剂量ＵＶＢ照射的角朊细胞更易表达Ｒｏ／ＳＳＡ抗原，提示ＵＶＢ和雌激素对角朊细胞表达Ｒｏ／ＳＳＡ抗原有协同作用。基于上述结果。
In order to investigate the mechanism of ultraviolet in triggering and exacerbating cutaneous lupus erythematosus,we conducted an in vitro experiment to study the effect of UVB irradiaton on the expression of Ro/SSA antigen on cultured human keratinocytes by indirect immunofluorenscence(IIF).The results revealed that the treatment of keratinocytes with serial doses of UVB (50mJ/cm 2, 100mJ/cm 2, 200mJ/cm 2) induced Ro/SSA antigen expression on the cell membrane.The Ro/SSA expression showed a dose dependent pattern with the maximal expression of 10 7% at the dose of 200mJ/cm 2. When 10 -5 mol/L 17 β estradiol was added to the culture medium 48 hours prior to 200mJ/cm 2 UVB exposure, it significantly increased Ro/SSA expression (17 2%) as compared with that induced by UVB alone(10 7%), which reveals that UVB irradiaton and estradiol treatment may bave synergetic action on the expression of Ro/SSA. From these results, we speculate that Ro/SSA expression on the surface of keratinocytes induced by UVB may function as an important trigger for antibody dependent keratinocyte damage in photosensitive cutaneous lupus erythematosus.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1997年第5期315-317,共3页
Chinese Journal of Dermatology
