摘要
为给防治放射性肺炎的药物研究提供实验依据，作者观察了富硒制剂对大鼠早期放射性肺炎的效果。方法雄性Ｗｉｓｔａｒ大鼠４８只分为对照组，单纯照射组，补硒照射组。γ射线照射，吸收剂量１５Ｇｙ，于０．５，１，２，３个月活杀取肺组织做光镜观察和肺组织ＲＮＡ提取，应用斑点杂交和图像分析技术测Ⅰ和Ⅲ型肺胶原ｍＲＮＡ含量。结果照射组大鼠肺损伤明显，而富硒制剂使肺充血、出血、渗出性变化及肺泡壁增厚程度减轻，且对肺Ⅰ（Ⅲ）型胶原ｍＲＮＡ合成有一定程度的抑制作用。结论富硒制剂对大鼠早期放射性肺炎有防治效果。
Objective In order to provide experimental basis for drug efficacy of preventing and treating radiation pneumonia,the authors observed the early effects of selenium preparation on the radiation pneumonia.Method Wistar male rats were divided into three groups of 48 rats:the control group,the irradiated group and the irradiated plus selenium preparation treated group.The animals received thoracic irradiation of 15 Gy,and were killed at 0.5,1,2,3 months post irradiation.Lung tissues were processed for light microscopy and isolation of RNA.Expression of type Ⅰ(Ⅲ) collagen was evaluated by dot blot hybridization and image analysis technique.Results Pathological study showed marked lung injury in the irradiated group while only slight hyperemia,hemorrhage,exudation and thickeness of alveolar walls in the lungs of selenium preparation treated group.The synthesis of type Ⅰ and Ⅲ collagen mRNA also decreased in the selenium preparation treated group.Conclusion Selenium preparation could have protective and therapeutic effects on radiation pneumonia.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1997年第2期88-90,共3页
Chinese Journal of Radiological Medicine and Protection
关键词
放射
肺炎
胶原
病理观察
富硒制剂
Radiation pneumonia Collagens Pathological study Selenium
