摘要
目的:探讨p63和p53基因在子宫内膜样腺癌(endometrial adenocarcinoma,EC)发生发展中的作用及其与EC临床病理特征的关系。方法:采用免疫组化EnVision法检测65例EC、25例子宫内膜增生过长(endometrial hyperplasia,EH)及15例正常增生期子宫内膜(benign proliferative endometrium,BPE)中P53和P63蛋白的表达情况。结果:P63蛋白在EC组中的阳性率为78.5%(51/65),与EH组(4/25,16%),BPE组(1/15,6.7%)比较,差异均有统计学意义(P<0.001);P63的表达与EC的组织学分级、手术病理分期和肌层浸润深度均无关(P>0.05)。P53蛋白在EC组中的阳性率为36.9%(24/65),明显高于EH(2/25,8%)组和BPE组(0/15)(P<0.01);P53的表达与EC的手术病理分期和肌层浸润深度显著相关(P<0.01),而与组织学分级无关(P>0.05)。P53和P63在子宫内膜样腺癌中的表达呈正相关(r=0.246,P=0.048)。结论:突变型p53和p63可能共同参与子宫内膜样腺癌的发生、发展,p63在EC的发生中可能起癌基因作用,其阳性表达可能是子宫内膜样腺癌发生的早期事件。
Objective：To study the roles of p63 and p53 in tumorigenesis and development of endometrial adenocarcinoma （EC） and their correlation with clinicopathologic features. Methods.The expressions of P63 and P53 were detected by the immunohistochemical stain in 65 cases of EC, 25 cases of endometrial hyperplasia （EH） and 15 cases of benign proliferative endometrium （BPE）. Results：The positive rate of P63 was 78. 5% （51/65） in EC, there was a significant difference between EC and EH （4/25, 16%） or BPE（1/15, 6.7%）,（P〈0. 001）. The expression of P63 was not associated with the histological grade, stage and depth of myometfial invasion in EC （P〉0. 05）. The positive rate of P53 was 36. 9%（24/65） in EC, and the expression was significantly higher than those in EH （2/25, 8%）and BPE（0/ 15）（P〈0. 01）. The expression of P53 was associated with the stage and depth of myometrial invasion in EC （P〈0.01）, but was not associated with the histological grade （P〉0. 05）. There was a significantly positive correlation between P63 and P53（r=0. 246, P=0. 048）. Conclusions： Mutant p53 and p63 are involved in carcinogenesis of EC, and there may be an assistant role between p53 and p63. p63 may be an oncogene in tumorigenesis of EC, and its over-expression may be an early event in development of endometrial adenocarcinoma.
出处
《医学理论与实践》
2008年第7期758-760,共3页
The Journal of Medical Theory and Practice
