摘要
目的建立盐酸阿夫唑嗪缓释片含量测定的高效液相色谱方法。方法色谱柱：Agilent Eclipse C18；流动相：四氢呋喃-乙腈-高氯酸钠溶液（将5mL高氯酸稀释到900mL水中，用NaOH溶液调pH值至3．5，加水至1000mL）=1：20：80；检测波长254nm。结果制剂中辅料和有关物质对主药无干扰，盐酸阿夫唑嗪在浓度15．0～35．5μg·mL^-1范围内线性关系良好，相关系数r=0．9999（n=5）；平均回收率（n=9）为100．68％（RSD=0．71％）。结论本法专属性好，准确，简便。
OBJECTIVE To establish an HPLC method for determination of alfuzosin in Alfuzosin Hydrochloride Sustained-release Tablets. METHODS The chromatographic column of Agilent Eclipse C18 was used, The mobile phase was consisted of tetrahydrofuran-acetonitrile-sodium perchlorate solution （Dilute 5mL perchloric in 900ml water,adjust the pH to 3.5 by dilute sodium hydroxide solution,and dilute the solution to 1000mL with water） = 1 ：20： 80. The detection wavelength was 254nm. RESULTS Alfuzosin and the other components in the samples were separated completely. There was a good linear relationship in the range of 15.0～35.5μg · mL^-1 with the correlation coefficient of 0. 9999. The average recovery of Alfuzosin tablets was 100.68% （ n = 9, RSD = 0.71 % ） respectively. CONCLUSION The method was specific,accurate and simple.
出处
《齐鲁药事》
2008年第6期349-350,共2页
qilu pharmaceutical affairs
