摘要
以富含次生代谢物的杜鹃红山茶嫩叶为材料,研究了杜鹃红山茶鲜叶保存方法及其基因组DNA提取方法。结果表明,改进的CTAB和SDS区室法都适合杜鹃红山茶基因组DNA的提取,能提取到完整性较好、纯度较高的基因组DNA。采用-20℃、液氮、硅胶脱水干燥3种方法保存样品,均可获得高质量DNA,说明硅胶脱水干燥法保存样品的方法可行,为远距离采样提取杜鹃红山茶DNA提供了一种较好的鲜叶保存方法。
The methods of preserving fresh leaves and genomic DNA extraction were studied in Camellia azalea, which are rich in secondary metabolites. Results showed a high-purity and a good-integrity DNA can be obtained from the preserved fresh leaves of Camellia azalea, using the methods of modified CTAB and SDS subarea. The DNA extracting quality from the leaves preserved with silica gel by the methods of modified CTAB and SDS subarea is high, which is the same as from the leaves preserved at the -20℃ and liquid nitrogen, so these preserving methods are suitable for the fresh leaves of Camellia azalea, including for it from distant place.
出处
《广东林业科技》
2008年第2期5-9,共5页
Forestry Science and Technology of Guangdong Province
基金
"十一五"国家科技支撑计划专题(2006BAD01A1802)
关键词
杜鹃红山茶
DNA提取
硅胶脱水干燥
Camellia azalea, DNA extraction, dehydrated desiccation with silica gel
