摘要
Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated that the specificity of immunofluorescence assay was high, and the positive predicted value was 100%, so it can be used as one of the confirmatory methods for HIV -1 antibody.
Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated that the specificity of immunofluorescence assay was high, and the positive predicted value was 100%, so it can be used as one of the confirmatory methods for HIV -1 antibody.
出处
《中国病毒学》
CSCD
1997年第4期371-373,共3页
Virologica Sinica
