摘要
采用组织学方法和电镜技术，对9个不同发育时期的鸡（Gallus domestiaus）胚胎进行了观察。通过对鸡胚胎肾组织发生过程的观察，探讨鸡胚中肾的发生与退化，后肾的发生、分化规律和特点。结果表明，孵育到第16期在中肾前端附近出现一些中肾小泡。孵育到第18期形成中肾小管。孵育到第26期，中肾小管的盲端内陷，原始的肾小囊和肾血管球形成，中肾小管显著伸长并迂回曲折。孵育到第33～37期，体前后部中肾组织均已形成完整的肾单位。第37～46期体前部至后部的中肾组织依次退化。孵育到第26期从泄殖腔附近发出的输尿管芽向生后肾组织侵入生长，生后肾组织产生许多生后肾小泡。第33期出现肾小囊和肾小管，肾小管伸长并发生折叠，出现集合小管、近端小管和远端小管的形态分化。第37～46期肾小体逐渐发育成熟，肾小管继续分化出现细段。鸡的中肾具有排泄功能。鸡后肾的发生与分化存在明显的时间差异肾单位的分化中，同一胚龄肾组织内可存在不同发育阶段的肾小体，集合小管分化较早，诱导近端小管和远端小管分化，细段分化较迟。
Electron microscopic and histological methods were applied to explore the development and degeneration of mesonephros, and the patterns and characteristics of metanephric development and differentiation. Mesonephros and metanephros were observed at 9 different incubation stages. Results showed that some mesonephric vesicles appeared in the vicinity of the anterior mesonephric ducts at the 16th stage. At the 18th stage mesonephric tubules took shape. At the 26th stage mesonephric tubules＇ blind ends invaginated, resulting in primitive renal sacs and glomernlus as well as mesonephric tubules elongated distinctly and curved back and forth. All mesonephric tissue had produced integrated nephric units during the 33rd - 37th stages. During the 37th - 46th stages mesonephric tissue from anterior to posterior of the embryos degenerated in turn. At the 26th stage of incubation, ureteric bud from cloaca grew into metanephrogenic tissue which produced many metanephrogenic vesicles. At the 33rd stage renal tubules and renal sacs appeared and renal tubules lengthened apparently and folded regularly, indicating morphological differentiation of collecting convoluted tubules, proximal convoluted tubules and distal convoluted tubules. During the 37th- 46th stages renal corpuscle developed and matured, and renal tubules produced functional differentiation graduall, indicating morphological differentiation the neck part of tubules. Mesonephros of chick embryo had excretory functions. Development and differentiation evidently vary with time changes at different sections of mesonephros. During development and differentiation of nephric units, renal corpuscles in different developing sections appeared at the same stage. The differentiation of collecting convoluted tubules was earlier, which induced the differentiation of proximal and distal convoluted tubules. The differentiation of neck part of tubules occurred later.
出处
《动物学杂志》
CAS
CSCD
北大核心
2008年第3期131-138,共8页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No.30370163)
甘肃省自然科学基金项目(No.ZS031-A25-005-Z)
甘肃省创新人才基金项目
关键词
鸡
中肾
后肾
胚胎发生
Chick
Mesonephros
Metanephros
Embryonic development
