摘要
目的探讨骨肉瘤中AQP1的表达和临床意义。方法对39例骨肉瘤和22例骨良性疾病对照组标本采用免疫组化法观察AQP1的表达并用人工计数和自动图像分析两种方法检测其表达差别,同时行CD34抗体染色标记肿瘤微血管,计算所有标本MVD。结果①AQP1表达于肿瘤微血管和小血管内皮细胞及绝大多数骨肉瘤的肿瘤细胞,尤其见于骨肉瘤的肿瘤巨细胞上;②人工计数AQP1阳性细胞百分比(YP)、AQP1人工计数分级、自动图像分析所得平均光密度(MD),均表明AQP1在骨肉瘤中高表达(P均<0.01);③骨肉瘤中AQP1的YP与MVD呈明显正相关(r=0.341,P<0.05)。结论大多数骨肉瘤肿瘤细胞高表达AQP1的原因不明;AQP1在骨肉瘤生物学行为及肿瘤微血管生成中占有重要作用;提示AQP1有可能作为骨肉瘤治疗的新靶点。
Objective To investigate the distribution and expression of aquaporin I（AQP 1） in osteosarcoma（OS） group and the control group, confirm the correlation between the expression and microvascular density（MVD）, and study its significance. Methods The expression of AQP1 was demonstrated through immunohistochemistry in both groups. Differences were detected by artif-counting and auto-image analysis. Microvessels in the tumor were marked with CD34 antibody simutaneously, and the MVD in all specimen was caculated. Results ①AQP1 was expressed highly in microvessels in the tumor, the endothelium in small vessels, and most osteocarcomas,.especially the osteosarcomatous tumor giant cells. ②The percentage of positive cells（YP）, the artif-counting gradation, the positive rate of MD shown through auto-image analysis all indicated higher expression in OS group than that in the control group （P〈0.01, P〈0.01, P=0.011）. ③In OS, positive correlation was found between YP of AQP1 and MVD（r=0.341, P〈0.05）. Conclusion The high expression of AQP1 in osteocarcomas still cannot be clearly explained. AQP1 plays an important role in osteosarcomatous biological behaviour and microvascularization in tumors. AQP1 may be a new target for osteosarcomatous therapy.
出处
《中国骨肿瘤骨病》
2008年第3期142-145,共4页
Chinse Journal Of Bone Tumor And Bone Disease
关键词
骨肉瘤
水通道蛋白1
肿瘤血管生成
微血管密度
Osteosarcoma（OS）
Aquaporin I（AQP1）
Tumor angiogenesis
Microvascular density（MVD）
