摘要
用25个微卫星标记,对5个山羊品种进行遗传多样性分析。共检测到199个等位基因,并进行哈代-温伯格平衡检验,5个群体基本处于遗传不平衡状态。在25个座位中,除BMS1943在罕山白绒山羊上（PIC为0.493）表现为中度多态外,其余座位均为高度多态。以等位基因为基础,得出座位的平均杂合度为0.436-0.820之间,群体平均杂合度在0.592-0.738。对各微卫星标记进行F-统计分析,Fst的变化范围是从BMC1206的0.011到BMS1943的0.078;群体每代迁移数在2.972-23.115,平均值为8.884。计算了遗传分化系数,结果表明群体间变异程度不高。内蒙的罕山白绒山羊和乌珠穆沁绒山羊有较大的基因流值（15.753）,说明其交流比较活跃。计算了共祖遗传距离,并进行了UPGMA和NJ聚类分析,内蒙的罕山白绒山羊和乌珠穆沁绒山羊聚到一起。
The genetic diversity of five goat breeds was surveyed using twenty-five microsatellite loci. 199 alleles were gotten in all the loci,and the Hardy-Weiberg equilibrium analysis revealed that almost the 5 breeds in the 25 loci showed a remarkable genetic disequilibrium. The PIC value （0. 493） were less than 0.5 in BMS1943, the other loci were high diversity. The mean heterozygosity of different locus varied from 0. 436 to 0. 820, and of different population was from 0. 592 to 0. 738. The F-statistic analysis indicated that the Fs, of different locus varied from 0. 011 （inBMC1206） to 0.078 （inBMS1943）. The Nm was from 2. 972 to 23.115,and the mean value was 8. 884. The result of the cofficient of gene differentiation showed it was not remarkable differentiation in all the breeds. Adopted the method of UPGMA with the Dcou genetic distance in the 5 goat breeds , the dendrograms were drawed, Hanshan White Cashmere goat and Wuzhumuqin Cashmere goat were in the close relationship each other.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第6期709-714,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
“十一五”科技支撑(2006BAD13B08)
“863”国家高技术研究与发展计划(2006AA102Z198)
国家科技基础条件平台项目(2005DKA21101)
中国农业科学院北京畜牧兽医研究所“家畜种质资源研究与创新”科技创新团队项目(ywf-td-1)
关键词
山羊
微卫星
遗传多样性
遗传距离
聚类分析
goat
microsatellite
genetic diversity
genetic distance
dendrograms
