摘要
针对牛肌肉生长抑制素基因（MSTN）3个外显子区设计引物,通过PCR扩增及测序,以蒙古牛、雷琼牛、独龙牛、巴音郭楞州牦牛共66个样本为材料检测了中国普通牛、瘤牛、大额牛和牦牛4个牛属该基因外显子区核苷酸序列的变异。结果显示,4个中国牛种的MSTN基因外显子1、2和3分别含375、372、381个碱基对,在所检样本中,发现7个核苷酸多态位点,定义17种单倍型;种内核苷酸歧异度（Pi）在0.000 36-0.001 03间,种间核苷酸歧异度（Dxy）在0.000 76-0.003 22间,说明群体内遗传多样性程度低;以单倍型序列为基础构建的分子进化树表明,牦牛的分化早于黄牛中普通牛和瘤牛的分化;大额牛明显分为2个类群,很可能是由于杂交造成的。
Through designing the primers to the three exons of the myostatin （MSTN） gene and using the methods of PCR amplification and sequencing, variations of the MSTN gene were detected in 4 Chinese Bos species, normal cattle （Mongolia cattle）, zebu （Leiqiong cattle）, yak （Bayingolin）, and gayal （Dulong gayal）. The results showed that 375,372,381 base pairs were detected respectively in the exon 1, 2 and 3 of the MSTN gene, and 7 nucleotide polymorphic sites were observed with 17 haplotypes in the 4 Bovinae populations. The nucleotide diversities within the 4 species （Pi） ranged from 0. 000 36 to 0. 001 03, and between the 4 species （Dxy） ranged from 0. 000 76 to 0. 003 22, indicating a low intrapopulation genetic diversity. Construction of the phylogenic tree based on these MSTN haplotypes indicated that yak was diverged earlier than yellow cattle （normal cattle and zebu） ; gayals were clustered into two groups, probably due to hybridization.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第6期701-704,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30571323)
