摘要
以鲁西牛、秦川牛、安格斯、海福特和西门塔尔与蒙古牛杂交牛5个品种共178头为试验材料,通过等位基因特异性聚合酶链式反应(AS-PCR)技术,发现牛乙酰辅酶A酰基转移酶1(ACAA1)基因的A1066G(GenBank登录号:EF576938)和A1452G(GenBank登录号:EF576938)2个单核苷酸多态(SNP),其中A1066G突变位于外显子9,是同义突变,A1452G突变位于3′非翻译区(UTR);发现牛乙酰辅酶A酰基转移酶2(ACAA2)基因的C33119T(GenBank登录号:EF583005)单核苷酸多态,位于内含子8上。对ACAA1基因的2个SNP进行分析没有发现单倍型。2个基因多态性与牛生长性状的关联分析表明:ACAA1基因A1066G突变位点的3种基因型间眼肌面积差异显著(P<0.05),AA型最高,AG型次之,GG型最低;ACAA1基因A1452G突变位点的3种基因型间眼肌面积差异显著(P<0.05),GG型最高,AG型次之,AA型最低;ACAA2基因C33119T突变3种基因型间日增重差异极显著(P<0.01),其中CT型最高,TT型次之,CC型最低;眼肌面积差异显著(P<0.05),其中CC型最高,TT型次之,CT型最低。
178 cattle from five breeds, Luxi, Qinchuan, Angus, Hereford and crossbreed of Simmental and Menggolian were used in the experiment. Through allele-specific polymerase chain reaction （AS-PCR）, two single nucleotide polymorphisms （SNP）. A1066G （GenBank Accession No. EF576938） and A1452G were found on ACAA1 gene. A1066G SNP is located at exon 9, but it is a silent mutation, while A1452G SNP is located at 3＇-untranslated region （UTR）. C33119T SNP （GenBank Accession No. EF583005） was found at intron 8 of ACAA2 gene. No haplotype was found between these two SNPs of ACAA1 gene. Results of association analysis of these SNPs with production traits showed that A1066G substitution of ACAA1 is associated significantly （P（0.05） with loin-eye area, the mean value of genotype AA is the highest, genotype AG second highest and genotype GG the lowest. A1452G substitution is associated significantly （P〈 0.05） with loin-eye area, the mean of genotype GG is the highest, genotype AG second highest and genotype AA the lowest. The C33119T substitution is associated significantly with daily gain （P〈0.01） and loin-eye area （P〈0.05）, for daily gain, the mean of genotype CT is the highest,genotype TT second highest, and genotype CC the lowest; for loin-eye area, the mean of geno type CC is the highest, genotype TT second highest, and genotype CT the lowest.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第6期696-700,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"十一五"国家科技支撑计划重大项目(2006BAD01A10)
