摘要
Switching Mechanism At 5′ end of the RNA Transcript(SMART)技术是一项应用于生物科学研究领域的方法,典型特点是能保证全长cDNA的获得。目前尚未见有关该技术的综述发表,系统阐述了SMART的原理和方法,综述了SMART技术的最新进展。SMART技术应用领域在不断扩大,不仅用于全长cDNA文库构建,而且也渗透到基因研究的诸多方面。虽然SMART技术是非常有效和实用的一项技术,但同时也存在一定问题需要改进。
Switching Mechanism At 5＇ end of the RNA Transcript（SMART） is a technology used in biology researching,So far, there is no review only about SMART technology. So,the aim is to investigate the research developments of principles, methods and applications of the SMART technology. Based on some researches and combined with the review of the related literature at home and abroad, it analyzed and evaluated the latest development of the research on SMART. As the applications of the SMART technology expand in many fields day after day, it has been proved that the SMART technology is a very useful and efficient skill to construct full length cDNA library. As more and more researchers know this technology, the advantages of the SMART technology become obvious, and meanwhile the disadvantages of the SMART technology also show up. That is to say, the technology needed to be improved.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第6期129-132,共4页
China Biotechnology
基金
国家自然科学基金资助项目(C03030303)
