摘要
以香榧优株茎段为外植体,进行腋芽诱导,结果表明,在B5+BA0.1mg/L+NAA0.5 mg/L+IBA1.0 mg/L的培养基中腋芽诱导效果最好,诱导率可达70%;腋芽继代培养较适培养基为MS+BA1.0 mg/L+NAA0.1 mg/L,继代培养20 d可长成4 cm左右的健壮无根苗。采用腹接法将组培得到的无根苗嫁接于当年播种的实生砧木上,腹接法60 d愈合率可达80%以上。
Tissue culture of stem from strong Torreya grandis cv. Merrillii tree as explants was completed for induction of axillary bud. The highest induction rate （70%） was obtained on B5 culture medium supplemented with BA0.1 mg/L＋NAA0.5mg/L＋IBA 1.0mg/L. And the appropriate medium for subculture was the MS supplemented with BA1.0mg/L＋NAA0.1mg/L. 20 days later, it could be cultured as healthy seedless seedling of about 4cm long. Then the seedling would be belly grafted to current seedling. 60 days later, the recover rate could be topped to more than 80%.
出处
《浙江林业科技》
北大核心
2008年第3期56-58,共3页
Journal of Zhejiang Forestry Science and Technology
基金
浙江省科技厅重点项目(2006C22086)
关键词
香榧
组培
嫩枝
嫁接
Torreya grandis cv. Merrillii
tissue culture
softwood
grafting
