摘要
应用反相高效液相色谱(RP-HPLC)法建立穿心莲药材指纹图谱。首先以穿心莲对照药材为对象建立其乙醇提取物特征色谱图,再用5种不同来源穿心莲药材的乙醇提取物验证色谱图的指纹特性。在穿心莲药材乙醇提取物特征色谱图中有共有峰10个,各共有峰的相对保留时间与相对峰面积的相对标准偏差(RSD)均小于4%。对照药材中共有峰峰面积占总峰面积的98.5%以上。该指纹图谱用于清火栀麦胶囊中穿心莲的鉴别专属性良好。
A fingerprint of standard raw material of Andrographis paniculata （Brum. f. ） Nees was developed by RP-HPLC technology. The enthanol extracts of Andrographis paniculata （Brum. f. ） Nees from different sources were analyzed by RP-HPLC, and it was found that the chromatograms of medicinal materials from 5 different sources were similar, specifically, there were 10 common characteristic peaks, and the peak area of the common peaks is beyond 98.5% of the total peak areas in the chromatogram of standard raw material of Andrographis paniculata （Brum. f. ） Nees.
出处
《分析科学学报》
CAS
CSCD
2008年第3期335-337,共3页
Journal of Analytical Science
基金
科技部攻关项目(No.2001BA701A01)
湖北省杰出青年基金(No.2002AC004)
关键词
穿心莲
高效液相色谱
指纹图谱
鉴别
Andrographis paniculata （Brum. f. ） Nees
HPLC
Fingerprint
Identification
