摘要
目的建立一种适合动态研究的大鼠深静脉血栓动物模型。方法将60只体重为(230±20)g的SD大鼠按随机数字表法分为3组:动物模型组36只,对照组18只,假手术组6只。动物模型组采用改良下腔静脉结扎法制备动物模型,造模后观察血管内膜及血栓不同时期的病理变化,并与对照组及假手术组做观察比较。结果采用改良法制作模型后,大鼠下腔静脉内不仅均形成稳定的血栓,而且形成血栓的血管段的近心端保持通畅,符合人体静脉血栓形成急性期的病理演变。结论成功制作适合动态研究的大鼠深静脉血栓动物模型。
Objective To establish a model of deep venous thrombosis （DVT） in rats for dynamic study of antithrombotics or thrombolysis on thrombosis. Methods SD rats （n=60） were randomly divided into thrombosis model group （n=36）, control group （n=18） and sham operation group （n=6）. An improved method was used to make the inferior caval vein ligated in SD rats of thrombosis model group. After operation, rats in thrombosis model group and control group were divided into 6 period groups, The changes of thrombus and internal surface of vessels in each period were observed in thrombosis model group and were compared with those in other two groups, respectively, Results Stable venous thrombus were observed in all inferior caval vein in thrombosis model group, and the proximal part of venous thrombus was unobstructed and consistent with the pathological change of venous thrombosis during acute stage in human body. Conclusion The DVT model in rats was successfully established, which maybe helpful for dynamic study of the effect of antithrombotics or thrombolysis on thrombosis.
出处
《中国普外基础与临床杂志》
CAS
2008年第6期408-411,共4页
Chinese Journal of Bases and Clinics In General Surgery
关键词
静脉血栓形成
动物模型
病理学
Deep venous thrombosis Animal model Pathology
