摘要
目的:观察不同浓度吡格列酮对大鼠骨髓源性内皮祖细胞(EPCs)增殖、凋亡和功能的影响,并探讨其可能的作用机制.方法:正常SD大鼠24只,随机分为A组(对照组)和B,C,D组[吡格列酮组剂量依次为10,20,40mg/(kg·d)],灌胃处理10d后断颈处死,密度梯度离心法获取骨髓单个核细胞,经鉴定后证实为EPCs.每组细胞培养4d后消化、计数并用完全培养液培养.24h后检测NO生成量,3d后检测凋亡情况,7d后检测增殖能力.结果:与A组相比,B,C,D组EPCs增殖能力明显增高[B,C,D组A490nm分别为(0.423±0.004),(0.680±0.008),(0.443±0.005)vsA组A490nm(0.143±0.006),P<0.01];凋亡程度降低[B,C,D组分别为(32.8±0.8)%,(30.9±2.3)%,(33.0±3.9)%vsA组(40.1±1.1)%,P<0.01];培养上清中NO浓度显著提高[B,C,D组分别为(29.2±3.7),(41.0±7.7),(28.7±6.4)μmol/LvsA组(18.9±1.4),P<0.05];与C组相比,B组和D组促进EPCs增殖能力弱(P<0.01),培养上清中NO浓度相对低(P<0.05).结论:不同浓度吡格列酮均能提高EPCs数量和功能;20mg/(kg·d)吡格列酮对EPCs的作用较强.
AIM： To investigate the effects of different doses of pioglitazone on the proliferation, apoptosis and function of endothelial progenitor cells （EPCs） derived from bone marrow. METHODS： Twenty-four SD rats were devided into 4 groups randomly. The rats in group A （control group） received normal saline by intragastric administration, the rats in group B, C and D received pioglitazone 10, 20, 40 mg/（kg · d） respectively for 10 d. Mononuclear cells were collected from rats＇ bone marrow by density gradient centrifugation, cultured with Medium 199, and were identified by UEA-I and ac-LDL staining, and the double positive ones were identified as EPCs. Once being harvested, EPCs of each group were calculated and cultured. After 24 h of incubation, NO secretion of EPCs was measured by the Griess assay with spectrophotometer. Being incubated for 3 d, apoptosis rate was detected by FCM. After 7 d of incubation, proliferation capacity was measured by MTT assay. RESULTS： Compared with group A, proliferation of EPCs increased[A490nm ： （0. 423± 0.004）, （0. 680 ±0. 008）, （0.443±0. 005） VS control （0. 143 ±0. 006 ）, P 〈 0. 05 ], the apoptosis rate decreased [ （32.8 ±0.8）%, （30.9 ±2.3）%, （33.0 ±3.9）% vs control （40. 1 ±1.1 ） %, P 〈 0.05 ] and the production of NO enhanced [μmol/L, （29.2±3.7）, （41.0±7.7）, （28.7±6.4） vs control （18.9±1.4）, P〈0.05] in the group B, C and D. Group C showed the strongest effects among the 3 pioglitazone groups. CONCLUSION： Pioglitazone can improve the proliferation and the function of EPCs. The medium dose of 20 mg/（ kg· d） of pioglitazone has better effects.
出处
《第四军医大学学报》
北大核心
2008年第11期982-984,共3页
Journal of the Fourth Military Medical University
关键词
吡格列酮
内皮祖细胞
糖尿病
pioglitazone
endothelial progenitor cells
diabetes mellitus
