摘要
本研究应用BALB/c裸鼠为移植模型探讨阵发性睡眠性血红蛋白尿症(paroxysmal nocturnal hemoglobin-uria,PNH)患者CD34+CD59+的增殖和重建造血的能力,为实现PNH患者自体骨髓移植或自体外周血造血干细胞移植提供实验依据。利用免疫磁珠双阳性分选法,从正常人及PNH患者骨髓中分离出两组CD34+CD59+细胞,分别进行体外扩增,并将体外扩增的正常人和PNH患者骨髓CD34+CD59+细胞分别输注给接受亚致死剂量照射的BALB/c裸鼠。应用流式细胞技术和DNA检测技术检测受鼠骨髓、脾脏和外周血中人CD45+细胞。结果表明:PNH组的CD34+CD59+细胞在体外生存、扩增能力似弱于正常人对照组。但CD34+CD59+细胞输注给受鼠后6周,在受鼠骨髓、脾脏和外周血中可检测到人CD45+细胞,PNH组受鼠CD45+细胞水平与正常人对照组相比,无统计学差异。结论:PNH患者CD34+CD59+细胞仍具有同正常人CD34+CD59+细胞相同的生物特性,能体外扩增并能保持正常干/祖细胞的特性。
This study was purposed to investigate the expansion and hematopoietic reconstitiction capability of CD34 ^＋ CD59 ^＋ cells from patients with paroxysmal nocturnal hemoglobinuria （PNH） by using BALB/c nude mice so as to provide experimental basis for clinical anto-BMT or auto-PBHSCT in patients with PNH. CD34 ^＋ CD59 ^＋ceils were selected from the bone marrow mononuclear cells in normal persons and PNH patients by immunomagnetic positive double sorting and were engrafted sublethally irradiated BALB/c nude mice. The human CD45^＋ cells in bone marrow, spleen and peripheral blood of recipient mice were detected by flow cytometry and DNA assay. The results showed that the CD34^＋ CD59 ^＋ cells in PNH patient group and normal person group could expanded ex vivo, but ex vivo expansion capability of CD34^＋ CD59 ^＋ cells in PNH patient group at day 7 seemed inferior to that in normal control. While CD34 ^＋ CD59 ^＋ ceils of PNH patients and normal persons were transfused into recipient mice, the human CD45 ^＋ cells could be detected in bone marrow, spleen and peripheral blood at 6 weeks after transfusion, but there was no statistical difference in counts of CD45 cells between 2 groups. It is concluded that CD34 ^＋ CD59 ^＋ cells from PNH patients may keep characteristics of normal hematopoietic stem cells, and possess ability to expand ex vivo and support hemopoiesis.
出处
《中国实验血液学杂志》
CAS
CSCD
2008年第3期561-564,共4页
Journal of Experimental Hematology
