摘要
目的探讨蓝光滤过型人工晶状体(intraocular lens,IOL)对含N-亚视黄基-N-视黄基乙醇胺(A2E)的人视网膜色素上皮(retinal pigment epithelium,RPE)细胞的细胞活力、氧化应激及分泌血管内皮生长因子(vascular endothe-lial growth factor,VEGF)、色素上皮衍生因子(pigmentep-ithelium derived factor,PEDF)的影响。方法体外培养含A2E的RPE细胞,用低强度蓝光持续照射,在光通路上置蓝光滤过型(AcrySof Natural)或紫外线阻挡型(AcrySof)IOL。细胞分为:A组(AcrySof Natural IOL,光照);B组(AcrySof IOL,光照);C组(光照,无IOL);D组(无光照,无IOL)。CCK-8检测细胞活力,流式细胞仪检测活性氧自由基(reactive oxygen species,ROS)水平,酶联免疫吸附法检测还原型谷胱甘肽(glutathione,GSH)、VEGF、PEDF含量。结果A、B、C和D组细胞活力分别为(76.9±3.9)%、(42.5±4.3)%、(35.2±4.1)%、(96.7±3.1)%;ROS分别为511.53±67.43、1011.15±174.88、1022.23±158.72、452.82±77.98;GSH分别为(15.34±4.77)μmol/L、(2.57±1.96)μmol/L、(1.58±1.13)μmol/L、(19.73±5.49)μmol/L;VEGF/PEDF比值分别为1.40、9.76、14.86、0.71。同B、C组相比,A组细胞活力和GSH含量明显提高,ROS含量和VEGF/PEDF比值明显下降(P<0.05)。结论蓝光滤过型IOL对A2E介导的RPE细胞蓝光损伤有保护作用,能够降低蓝光诱导的ROS和VEGF水平,优于紫外线阻挡型IOL。
Objective To investigate the effect of a blue light-filtering intraocular lens （IOL） and an ultraviolet （UV）-absorbing IOL on blue light-induced damage to retinal pigment epithelial （RPE） cells laden with the lipofuscin fluorophore A2E, focusing on cell viability, the level of reactive oxygen species （ROS）, free glutathione （GSH）, vascular endothelial growth factor （VEGF）, and pigment epithelium-derived factor （PEDF）. Methods The A2E-laden RPE cells were exposed to blue light and a blue light-filtering IOL or UV-absorbing IOL was placed over the light beam. RPE cells were divided into： group A （AcrySof Natural IOL, light）; group B （AcrySof IOL, light）; group C（light,without IOL）; group D （without light and IOL） o After 60 hours of irradiation, the viability of the cells was determined with CCK-8 assay, the level of ROS was determined by flow cytometer, and the level of GSH, VEGF, PEDF were determined by enzyme-linked immunosorbent assay. Results Cell viability of A, B, C and D group was （76.9± 3.9）%, （42.5±4.3）%, （35.2±4.1）%, （96.7±3.1）% respectively; ROS was 511.53±67.43, 1011.15±174.88, 1022.23±158.72, 452.82±77.98 respectively; GSH was （15.34±4.77）μmol/L, （2.57± 1.96）μmol/L, （ 1.58±1.13 ）μmol/L, （ 19.73±5.49）μmol/L respectively; VEGF/PEDF ratio was 1.40, 9.76, 14.86, 0.71 respectively. The presence of the blue-light filtering IOL significantly attenuated light induced cell damage, increasing GSH and PEDF levels, de- creasing ROS and VEGF. Conclusion This study suggests that a blue light-filtering IOL may be more protective against A2E-in- duced blue light damage and inhibit more light-induced ROS and VEGF production than a conventional UV-absorbing IOL.
出处
《眼视光学杂志》
2008年第3期178-181,185,共5页
Chinese Journal of Optometry & Ophthalmology
基金
高等学校博士学科点专项科研基金(新教师基金课题)资助项目(20070246181)
