摘要
采用双抗夹心ELISA、APAAP桥联酶标法、MTT比色法检测了34例ITP患儿的血清可溶性白介素2受体（sIL-2R）外周血单个核细胞（PBMC）经PHA刺激后膜白介素2受体（mIL－2R）的表达、T淋巴细胞亚群以及自然杀伤（NK）细胞活性。结果：ITP患儿血清sIL－2R水平升高，mIL－2R的表达和NK活性显著降低；血清sIL－2R水平与NK活性、CD4+／CD8+比值是负相关，与疾病严重程度和疗效密切相关。提示sIL－2R可作为监测病情、观察疗效的敏感指标；在纠正ITP的免疫调节紊乱中应充分考虑到细胞因子受体异常这个环节。
We investigated the serum levels of soluble interleukin-2 receptor (mIL-2R ), the exprexssion of IL-2 receptor (mIL-2R ) on the peripheral blood mononuclear cells (PBMC), T lymphocyte subsets and NK cell activity in 34 0children with ITP and 19 healthy children. Serum levels of sIL-2R were significantly higher, but the mIL-2R expression and NK cell activity were decreased significantly (P <0. 01 ) in children with ITP as compared with healthy controls.Negative correlations were found between serum sIL-2R levels and NK cell activity,and between sIL-2R levels and the ratio of CD4+/CD8+. sIL-2R was associated with disease conditions and the effect of therapy. We concluded that sIL-2R measurement may consider as a sensitive marker of disease condition and response to therapy in patients with ITP. Our investigations indicatd that abnormality of cytokine receptor would be considered in correcting immunoregulation disorder in ITP.
出处
《临床血液学杂志》
CAS
1997年第4期155-157,共3页
Journal of Clinical Hematology
关键词
紫癜
血小板减少性
自然杀伤细胞
SIL-2R
Idiopathic thrombocytopenic purpura Interleukin-2 receptor Natural killer cell
