摘要
选取萌发3～5d、长度3～5cm的中国菟丝子(Cuscuta chinensis Lam.)幼苗,将其分为上、中、下3部分并作为外植体进行离体培养与植株再生研究。结果表明,其上、中部片段更适宜愈伤组织诱导;诱导培养基以添加1mgL-1NAA和1mgL-1BA的MMS培养基效果最好,此培养基也可用于愈伤组织的继代培养,愈伤组织在上述培养基中已生长一年之久。分化培养基为添加1mgL-1BA的MMS培养基,平均每块愈伤组织可以产生2.8株植株。
An efficient method of callus induction and plant regeneration of Cuscuta chinensis was established. The seedlings germinated for 3 -5 d and about 3 -5 cm length were selected as explants, and then divided into three parts, upper, middle and lower. Callus were inducted from upper or middle parts of seedlings on a modified Murashige and Skoog （MMS） medium supplemented with 1 mg L^-1 NAA and 1 mg L^-1 BA. The calli have been subcultured on such medium for over a year. Shoot regeneration from callus was achieved on MMS medium containing 1 mg L^-1 BA and could obtain 2.8 shoots per callus.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2008年第3期279-282,共4页
Journal of Tropical and Subtropical Botany
