摘要
用5种浓度的十二烷基苯磺酸钠(sodiumdodecyl benzene sulfonic,SDBS)浸泡刺激奥尼罗非鱼(Tilapianilotica♀×T.aurea♂)8周,每2周采血样1次,测定免疫指标。0.05和0.10mg·L-1组对奥尼罗非鱼的氯化硝基四氮唑兰(nitro blue tetrazolium,NBT)阳性细胞和IgM含量无影响。0.40mg·L-1组在第8周时,IgM含量显著下降,而NBT阳性细胞突然急剧下降,与对照组相比差异极显著。0.70mg·L-1组从第4周起,NBT阳性细胞和IgM含量开始减少,第6周时差异显著。1.00mg·L-1组NBT阳性细胞数在第2周时量就已经有减少,但与对照组相比差异不显著,到第4周时差异极其显著;在第4周时IgM含量有显著差异,第6周后差异极显著。攻毒试验表明,0.40、0.70和1.00mg·L-1组的死亡率均高于对照组。以上结果表明,当SDBS浓度大于0.40mg·L-1时,奥尼罗非鱼免疫功能不同程度受到抑制,对NBT阳性细胞和IgM的影响存在着时间与剂量效应。
The main immune indexes of hybrid tilapia （ Tilapia nilotica ♀× T. aurea ♂ ） were measured every two weeks. The 0.05 and 0. 10 mg·L^-1 sodium dodecyl benzene sulfonic （SDBS） had not affected the numbers of NBT-positive cells and content of IgM. The 0.40 mg·L^-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in eighth week. The 0. 70 mg·L^-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in sixth week. The 0. 10 mg·L^-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in fourth week. Mortality of hybrid tilapia after injection of Aeromonas hydrophila （0. 40, 0. 70 and 1.00 mg·L^-1 SDBS） was higher than the control. These results indicated that low concentration of SDBS （0. 05 and 0. 10 mg·L^-1） did not affect the immune function of hybrid tilapia, but the higher concentrations of SDBS （0.40, 0. 70 and 1.00 mg·L^-1 ） will apparently reduce the immune function of hybrid tilapia along with time changed.
出处
《南方水产》
CAS
2008年第3期33-37,共5页
South China Fisheries Science
