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一株环己酮高效降解菌株的筛选及鉴定

Screening and Identification of a High-Efficiency Cyclohexanone-Degrading Strain JDM-3-11
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摘要 自岳阳巴陵石化公司环己酮生产车间总出水口污泥中分离得到一株能快速降解环己酮的菌株,菌号为JDM-3-11.通过形态观察、生理生化特征检测和基于16SrRNA基因序列的系统发育分析,初步鉴定其为赤红球菌(Rhodococcus ruber)的一个菌株.当环己酮的质量浓度为2000mg/L时,在温度为30℃,转速为150r/min,pH值为7的条件下,70h内该菌株对环己酮的降解率达到94.79%. This paper reported screening a strain numbered JDM-3-11 for effectively degrading cyclohexanone, which was acclimated and isolated from the sludge from Baling Petrochemical Company in Yueyang. The strain was tentatively identified as Rhodococcus tuber by bacterial morphological observation, physiological and biochemical test, and 16S rRNA sequences analysis. The study showed that when the content of cyclohexanone was 2000 mg/L, the temperature was 30 ℃, the rotate speed was 150 r/min and pH was 7, the cyclohexanone biodegrading rate of strain JDM-3-11 within 70 hours reached 94.79 %.
出处 《吉首大学学报(自然科学版)》 CAS 2008年第2期92-95,116,共5页 Journal of Jishou University(Natural Sciences Edition)
关键词 环己酮 生物降解 16S RRNA基因序列 赤红球菌(Rhodococcus ruber) cyclohexanone biodegradation 16S rRNA gene sequence Rhodococcus ruber
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  • 1李舂华.[D].辽宁:辽宁石油化工大学,2003.
  • 2[2]Kuba T,Van Loosdrecht MCM,Heijne JJ.Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two ~ sludge system.Wat Res,1996,30:1702-1710.
  • 3[3]Simpson HD,Alphand V,Furstoss R.Microbiological transformations-49.Asymmetric biocatalysed Baeyer-Villiger oxidation:improvement using a recombinant Escherichia coli whole cell biocatalyst in the presence of anadsorbent resin.J Molecular Catalysis B:Enzymatic,2001,16(2):101-108.
  • 4[6]Doig SD,Avenell PJ,Paul AB,et al.Reactor operation and scale-up of whole cell baeyer-villiger catalyzed lactone synthesis.Biotech Prog,2002,18:1039-1046.
  • 5[7]Mari(e)t J,Van DERW.Purification and characterization of a Baeyer-Villiger mono-oxygenase from Rhodococcus erythropolis DCL14 involved in three different monocyclic monoterpene degradation pathways.J Biochem,2000,347:693-701.
  • 6[8]Sebastian R,Ulrich SL.Synthesis of chiral-lactones in a twoenzyme system of cyclohexanone mono-oxygenase and formate dehydrogenase with integrated bubble-free aeration Tetrahedron:Asymmetry,1997,8:2523-2526.
  • 7[9]Carballeira JD,Alvarez E,Sinisterra JV.Biotransformation of cyclohexanone using Geotrichum candidum NCYC49.Factors affecting the selectivity of the process.J Molecular Catalysis B,Enzymatic,2004,28,25-32.
  • 8[10]Lphand V,Archelas A,Furstoss R.Microbiological transformation 13.A direct synthesis of both S an R enantiomers of 5-hexadecanolide via an enantioselective microbiological Baeyer-Villiger reaction.J Org Chem,1990,55:347-350.
  • 9蒋文强,生物医药色谱进展,1996年,48页
  • 10匿名著者,空气和废气监测分析方法,1990年,220页

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