摘要
目的:通过体外实验评价紫杉醇对p53突变型肺癌细胞株H322的生长抑制作用。方法:紫杉醇不同作用浓度不同作用时间处理H322细胞后,应用MTT法检测紫杉醇对肺癌细胞生长抑制作用,通过流式细胞术分析细胞周期及凋亡的变化,荧光显微镜观察细胞核形态的变化。结果:紫杉醇对H322的细胞毒性呈时间依赖性(P<0.05),(10-1000)nmol/L的浓度对H322生长抑制差异不显著(P>0.05)。浓度≤100nmol/L时,G2-M期比率随浓度增加而增高(P<0.05);凋亡比例在低浓度范围内(0.1-10)nmol/L呈浓度依赖性,浓度进一步增加,反而降低。时间依赖性实验中,10nmol/L作用时,凋亡发生呈时间效应;G2-M期所占比例在12h达最高。1000nmol/L作用时,G2-M期阻滞程度和持续时间均高于10nmol/L,但不如低浓度诱导凋亡出现得早。典型凋亡细胞出现浓缩、成片的染色质。紫杉醇诱导的凋亡和G2-M期阻滞无相关性(P>0.05)。结论:体外实验中,紫杉醇对H322细胞有明显生长抑制作用,并且这种作用呈时间依赖性,但在一定浓度范围内剂量效应不明显;低浓度紫杉醇虽然对G2-M期阻滞影响较小,却能比高浓度更早诱发凋亡,延长作用时间亦可达到有效抑制肿瘤细胞增殖的目的。
Objective：To assess the growth -inhibiting effects of paclitaxel on human lung cancer H322 cell line in vitro. Methods： H322 cells were exposed to paclitaxel at different concentration and different treating time, then the growth inhibiting effects of the cell line were detected with MTT assay, and the change of the cell cycle and apoptosis were analyzed with flow cytometry. Fluorescence microscopy was used to observe the nucleolus change stained by Hoechst33342. Results： The growth - inhibiting effect of paclitaxel on H322 cells was time dependent（P 〈 0.05）. The inhibition effects caused by paclitaxel from 10nmol/L to 1000nmol/L had not significant difference（P 〉 0.05 ）. At the concentration -dependent experiment, the proportion of apoptosis was highest at 10nmol/L paclitaxel, while the percentage of G2 - M phase increased in accordance with raise of the paclitaxel concentration, when paclitaxel concentration changed from 0.1 nmol/L to lOOnmol/L（P 〈 0.05）. At the time - dependent experiment, the inducement of apoptosis was time dependent（P 〈0.05） and the percentage of G2 - M phase was highest at 12h when pacli- taxel was 10nmol/L. Compared to 10nmol/L paclitaxel, 1000nmol/L paclitaxel could induce a higher degree and du- ration of G2 - M arrest and a later apoptosis. Apoptosis had no relationship with G2 - M arrest（P 〉0.05）. The typical apoptotic cells with condensed and fragmented chromatin could be observed in cells treated by paclitaxel under Fluorescence microscopy. Conclusion： Paclitaxel inhibits the growth of H322 cells in vitro in time - dependent manner. It can induce apoptosis and G2 - M arrest but the later two have no relationship between each other. Low con- centration paclitaxel can also inhibit effectively the growth of H322 ceils by prolonging treatment.
出处
《现代肿瘤医学》
CAS
2008年第6期900-903,共4页
Journal of Modern Oncology
