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小细胞肺癌多药耐药细胞差异表达片段磷酸二酯酶2A(PDE2A)在多种肿瘤细胞中表达的研究 被引量：1

The expression of PDE2A of the differentially expressed cDNA fragments of human small-cell lung cancer (SCLC) multi-drug resistance (MDR) cell in various tumour cells
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摘要 目的:检测PDE2A在多种肿瘤细胞中的表达情况,以了解PDE2A基因的表达分布特点及初步功能特征,为进一步肿瘤多药耐药相关研究奠定基础。方法:利用Northern杂交与RT-PCR方法检测PDE2A在H446、H446/CDDP、A549、A549/CDDP、SK-HEP-1、Namalwa、SGC7901等7种肿瘤细胞中的表达情况。结果:PDE2A仅在H446/CDDP中表达,而在其他细胞中无表达。结论:在所检测的7种肿瘤细胞中,PDE2A在H446/CDDP中高表达。PDE2A可能参与了H446/CDDP多药耐药性的形成。 Objective：To detect the expression of PDE2A in SCLC MDR cell and various other tumour cells and to investigate the distribution and function characteristics of the PDE2A expression. Methods： RT-PCR and Northern blot were used to detect the expressions of PDE2A in seven tumour cells including H446, H446/CDDP, A549, A549/CDDP, SK-HEP-1, Namalwa and SGC7901. Results： Semi-quantitative RT-PCR and Northern blot showed that PDE2A only had expression in H446/CDDP. Conclusion： Gene PDE2A has different expressions in SCLC MDR cell H446/CDDP; the gene might participate in the formation of the MDR in H446/CDDP.
作者 李昆霖 吴国明 戢福云 徐智 黄桂君
机构地区 第三军医大学新桥医院全军呼吸内科研究所
出处 《现代肿瘤医学》 CAS 2008年第6期880-882,共3页 Journal of Modern Oncology
基金 国家自然科学基金资助项目(编号:30200119)
关键词 小细胞肺癌 多药耐药 磷酸二酯酶2A small cell lung cancer MDR phosphodiesterase 2A
分类号 R73-3 [医药卫生—肿瘤] R734.2 [医药卫生—肿瘤]
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参考文献11

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二级参考文献9

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现代肿瘤医学
2008年 第6期

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