摘要
目的:研究探讨新西兰大白兔角膜碱烧伤后LYVE-1(淋巴管内皮透明质酸盐受体1)的表达及意义,进一步证实角膜新生淋巴管的存在,揭示其与角膜碱烧伤损伤修复的关系。方法:饲养24只健康新西兰大白兔,左眼制作角膜碱烧伤模型作为实验组,右眼不做手术作为对照组。术后1天、3天、7天、14天分别处死6只兔,免疫组化测定两眼LYVE-1的表达情况。结果:实验组于术后第3天出现表达,第7天、第14天表达增加;实验组术后3天、7天和14天LYVE-1表达均高于对照组。结论:角膜新生淋巴管在角膜碱烧伤中扮演重要角色。
Objective ： To examine the expression of LYVE - 1 in the cornea of New Zealand rabbit undergoing alkali burn so as to confirm the onset of corneal neolymphangiogenesis as well as its correlation with post - burm corneal restoration. Methods ： The left eyes of 24 healthy New Zealand rabbits were all subjected to alkali burn as the experimental group, while the untreated right eyes were chosen as the control group. Every six rabbits chosen randomly were killed at t = 1 day, 3 day, 7 day and 14 day after operation respectively. The corneal expression of LYVE - 1 was then examined through immunohistochemistry method. Resuit： LYVE - 1 expression in the experimental group exhibits progressive increase starting from the third day after operation and reaches its peak presumably around the 7th day after operation. LYVE- 1 expression in the experimental group exhibits much higher level than the control group at each time point （ t =3 day, 7day and 14 day） examined. Conclusion： Corneal neolymphangiogenesis plays an important role in post - burn corneal restoration.
出处
《包头医学院学报》
CAS
2008年第3期240-241,共2页
Journal of Baotou Medical College
