摘要
目的:探讨环孢素A(CsA)、维拉帕米(VP)、川芎嗪(TMP)对胃癌多药耐药细胞株SGC-7901/ADR耐药性的逆转作用。方法:采用四甲基偶氮唑盐(MTT)比色法观察SGC-7901/ADR对化疗药氟尿嘧啶(5-FU)的耐药性,选择CsA、VP、TMP的非细胞毒性剂量,并观察其与5-Fu联合对SGC-7901/ADR的逆转作用。结果:SGC-7901/ADR对化疗药5-FU具有耐药性,相对耐药性(RF)为97.49;CsA 3.0mg/L、VP 10.0mg/L、TMP 300.0mg/L以下为SGC-7901/ADR细胞的非细胞毒性剂量,除CsA外,VP和TMP对SGC-7901/ADR的逆转作用呈剂量依赖关系;300.0mg/LTMP较10.0mg/L VP逆转作用强(P<0.01),使SGC-7901/ADR相对耐药性降至12.89,将5-FU IC50由13.001mg/L下调到1.542mg/L,逆转倍数是8.43倍。结论:SGC-7901/ADR对5-FU具有耐药性,300.0mg/L TMP是SGC-7901/ADR细胞多药耐药性最有效的逆转剂。
Objective： To investigate the reversal effect of Cyclosporin A （CsA） , Verapamil （ VP ）, and Tetramethylpyrazine （TMP） against the multi drug resistance （MDR） gastric cancer cell line SGC -7901/ADR. Methods： MTr assay was used to assess the sensitivity of SGC -7901/ADR cells to Fluorouracil （5-FU）. CsA, VP, and TMP at non-toxic doses were separately applied together with 5 - FU to examine potential reversal effect against SGC - 7901/ADR cells by each agent. Resuits： SGC-7901/ADR cells are resistant to 5-FU with relative resistance （RF） of 97.49. The non-cytotoxic dose limits for CsA, VP, and TMP are 3.0 mg/L, 10.0 mg/L, and 300.0 mg/L respectively. Both VP and TMP but not CsA exert dose - dependent reversal effect against SGC -7901/ADR cells with TMP at 300.0 mg/L exerting stronger reversal effect comparing with VP at 10.0mg/L （ P〈 0.01 ）. At 300.0 mg/L, TMP reduces the RF of SGC - 7901/ADR cells to the value of 12.89 and lowers the IC50 of 5 -FU from 13. 001 mg/L to 1. 542 mg/L with a reversal fold of 8.43. Conclusion： Multi drug resistance cell line SGC - 7901/ADR is resistant to 5 - FU while TMP at 300.0 mg/1 is the most effective reversal agent examined here against SGC - 7901/ADR cells.
出处
《包头医学院学报》
CAS
2008年第3期230-232,共3页
Journal of Baotou Medical College
