摘要
目的观察硫酸乙酰肝素蛋白聚糖(HSPG)对C3H小鼠乳腺癌移植瘤的抑制作用及其机制。方法建立肿瘤模型并随机分为5组:生理盐水组、HSPG组(5、10、50mg.kg-1)及阳性对照组,各组动物自模型建立d2开始腹腔注射给药治疗22d并观察记录肿瘤体积。d24处死动物,称量肿瘤重量,计算胸腺指数及脾指数,使用原位凋亡检测法(TUNEL)检测HSPG对肿瘤细胞凋亡的影响,免疫组化检测血管内皮生长因子(VEGF)的表达。结果HSPG可明显抑制肿瘤生长,而不降低胸腺指数和脾指数。TUNEL结果HSPG组肿瘤组织中出现大量蓝黑色凋亡细胞,且明显多于生理盐水组,HSPG组肿瘤组织的VEGF的表达明显降低。结论HSPG对C3H小鼠移植性乳腺癌生长具有明显的抑制作用,其机制可能与诱导肿瘤细胞凋亡及抑制VEGF表达有关。
Aim To observe the anti-tumor activity and the mechanism of heparan sulfate proteoglycan （HSPG） on C3H mice transplanted tumors. Methods Tumor model was established and randomly divided into five groups. HSPG groups （5, 10, 50 mg · kg^-1）, positive group and control group, intraperitoneal injection was performed once a day for 22 days and the volume of tumors was measured. Mice were treated on 24th day, then tumor weight was examined, thymus index, and spleen index were calculated, the apoptosis was determined by TdT-mediated Dutp nick end labeling （TUNEL） assay in situ, the expression of vascular endothelial growth factor （VEGF） was detec- ted by immunohistochemistry. Results The tumor volume in HSPG groups was reduced without the decrease of thymus index, spleen index. TUNEL assay in situ sh HSPG groups cantly group. owed numerous heavy blue apoptosis cells in the groups significantly higher than in control The tumors in HSPG groups showed signifilower VEGF expression than those in control Conclusion HSPG had significant anti-tumor effects on C3H mice transplantable breast cancer. The mechanisms may be associated with the effects of inducing tumor cell apoptosis and inhibiting the VEGF expression.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第6期744-748,共5页
Chinese Pharmacological Bulletin
基金
安徽省人才开发资金资助项目(No2002Z023)
