摘要
目的研究全反式维甲酸(ATRA)与苯那普利对肾硬化大鼠肾脏组织α-SMA表达的影响。方法80只Wistar♂大鼠随机分为假手术组、模型组、苯那普利组和ATRA组,每组均为20只。采用单侧肾切除加1wk后尾静脉注射阿霉素(5mg·kg-1)的方案建立大鼠肾小球硬化(GS)模型,造模后12wk末处死。HE染色观察肾脏病理改变,计算肾小球硬化指数(GSI)。RT-PCR结合免疫组织化学方法检测肾组织α-平滑肌肌动蛋白(α-SMA)的表达情况。结果与模型组相比,ATRA组和苯那普利组GSI、α-SMA mRNA相对表达量和α-SMA阳性面积比均明显下降(P<0.05),但两组间各项指标比较无差异(P>0.05)。结论ATRA与苯那普利对大鼠肾硬化均有明显的延缓效应,且疗效一致。
Aim To investigate the effects of all-trans retinoic acid and benazepril on the expression of orsmooth muscle actin in rats with glomerulosclerosis. Methods 80 Wistar male Rats were randomly as- signed into the following groups： control group, model group, ATRA treatment group and benazepril treatment group, 20 rats in each group. GS rats were uninephrectomized and injected with adriamycin （5 mg·kg^-1） after one week through the tail vein. All rats were sacrificed at the 12th week, GS was evaluated by glomerulosclerosisindex （GSI） system. The expression of α-SMA was assessed by reverse transcription-poly-merase chain reaction （ RT-PCR ） and immunohisto- chemistry. Results Comparing with control group, the expression of ct-SMA mRNA and protein were de- creased significantly in ATRA treatment group and benazepril treatment group （ P 〈 0. 05 ）, but obvious differences of every index were not observed between the two groups （P 〉 0. 05 ）. Conclusions The post- poned effects of ATRA and dent and equivalent. benazepril on GS were evi-
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第5期659-663,共5页
Chinese Pharmacological Bulletin
基金
广西自然科学基金资助项目(No0640103)
