摘要
目的:建立测定黄药子配伍当归中主要成分含量的分析方法,比较黄药子配伍当归合煎液与单煎混合液中阿魏酸和黄独乙素的含量差异。方法:采用HPLC/PDA方法,对黄药子配伍当归合煎液与单煎混合液中的上述2个主要成分进行含量测定。应用Kromasil C18柱(250mm×4.6mm,5μm),0.1%乙酸水与纯色谱甲醇进行梯度洗脱,流速为0.8ml.min-1,检测波长300nm,柱温为30℃,进样量为10μL。结果:这2个化合物在测定的范围内表现出良好的线性(r>0.9995),方法的回收率在93.7%～102.8%之间。黄药子配伍当归合煎液中阿魏酸的含量高于单煎混合液,而黄独乙素在黄药子配伍当归合煎液中的含量低于单煎混合液。结论:黄药子配伍当归合煎可提高有效成分的溶出,降低有毒成分的含量。
Object:To develop a method for the determination of ferulic acid and Diosbulbin B in Dioscorea bulbifera L.and Angelica sinensis Diels decoction,and analyze the contents of these two compounds in co-boiling and pooled in-dividable boiling extracts.Method:An HPLC/PDA method was applied with KromasilC18 column.Gradient elute was performed by the mobile phrase consisted of 0.1 % acetic acid and color spectrum methanol with the flow rate of 0.8 mL·min-1.The column temperature was set at 30℃ and the detection was set at UV 300 nm,the sample injection volume was 10 μL.Results:The recovery of the method was in the range of 93.7%～102.8%,and all the compounds showed good linearity(r>0.9995) in a detected concentration range.The contents of two compounds were different in co-boiling and pooled individual boiling extracts.Conclusion:The contents of ferulic acid increase and toxic Diosbulbin B decrease significantly after boiling the individual crude drugs together.
出处
《中医药信息》
2008年第3期60-62,共3页
Information on Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30572368)
关键词
黄药子配伍当归
含量
HPLC/PDA
Dioscorea bulbifera L.and Angelica sinensis Diels Decoction
Contents
HPLC/PDA
