摘要
[目的]通过能力验证提高微生物实验室检测能力,检验人员技术水平,增强实验室竞争力。[方法]依据国家标准GB/T 4789-2003中对沙门氏菌、致病性大肠埃希氏菌检测方法结合沙门菌,大肠杆菌O157∶H7显色培养基及ATB生化鉴定仪对样品进行鉴定。[结果]经分离鉴定,PTA 101样品中检出2株沙门菌,分别是巴拿马沙门菌和肯塔基沙门菌;PTB077样品中检出大肠杆菌O157∶H7,而PTA385和PTB260中未检出目标菌。[结论]本次能力验证使用选择性强,特异性高的显色培养基及快速生化鉴定条(ATBID32E),克服了国标中致病菌检测周期长的特点,使检测更加快速、特异、敏感,取得满意结果。
[Objective]Ability test can improve microbiotic laboratory detection capability,laboratory technician levels and increasing laboratory competition. [Methods]According to international standard GB/T 4789-2003, Samples were detected via international standard methods combined with their own chromogenic medium and ATB biochemistry identification cards. [Results]Via isolation and identification two kinds of salmonla were isolated from PTA 101 samples, they are s. panama and s. Kentucky seperately; Enterohemorrhagic escherichia coli O157 ： H7 were isolated from PTB077 sampies,while PTA385 and PTB260 hadn＇t been detected objective bacterium. [Conclusion]This ability test we use medium with high selection and sensibility, ATB ID32E made detection more rapid,special and sensibility compared with international standards. The test results is satisfactoried.
出处
《预防医学论坛》
2008年第5期426-427,共2页
Preventive Medicine Tribune
关键词
能力验证
沙门菌
大肠杆菌O157∶H7
Ability test
Salmonla
Enterohemorrhagic escherichia coli O157 ： H7
