摘要
本文研制了鼠抗人Ⅳ型胶原单克隆抗体与兔抗人Ⅳ型胶原多克隆抗体，用双抗体夹心法建立了人血清Ⅳ型胶原的酶联免疫吸附试验（ＥＬＩＳＡ）检测法。本法灵敏度为１６ｎｇ／ｍｌ，批内变异系数为１０．０２％，批间变异系数为９．１４％，回收率为９２．６８％。与Ⅰ型胶原无交叉反应。经５０例健康成人血清Ⅳ型胶原浓度测定，其范围在５～７３ｎｇ／ｍｌ，均值为３１．０６±１８．８１ｎｇ／ｍｌ。临床初步应用于肝硬化及肝癌患者血清Ⅳ型胶原的测定，其均值分别为８７．２７±５１．０８ｎｇ／ｍｌ及１４６±５８．８６ｎｇ／ｍｌ，具有一定的诊断意义。
Monoclonal antibody and polyclonal antibody to human Type Ⅳ collagen were produced by hybridoma technology and conventional immunization.A sandwich type of enzyme linked immunosorbent assay for serum human typeⅣ collagen was established.The sensitivity of the immunoassay was 16ng/ml.The CV value of intra assays was 10.02%,of inter assays was 9.14%.The accuracy was 92.68%.No cross reaction was found with Type Ⅰ collagen.The concentrations of serum TypeⅣ collagen from 50 adult healthy humen were 31.06± 18.81ng/ml with a range of 5～73ng/ml.Serum samples from patients with liver cirrhosis and cancer showed increased value of serum TypeⅣ collagen concentration than that from the healthy controls in a very significant degree.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1997年第4期266-268,共3页
Immunological Journal
关键词
Ⅳ型胶原
ELISA
诊断
肝硬变
肝肿瘤
Type Ⅳ collagen,Enzyme linked immunosorbent assay,Hepatic fibrosis
