摘要
目的探讨多药耐药（MDR）基因产物多药耐药相关蛋白（MRP1）、肺耐药蛋白（LRP）和谷胱甘肽S-转移酶-π（GST-π）在胆囊癌和胆管癌中的表达及其与耐药的关系。方法采用免疫组化的方法（IHC）检测实验组18例胆囊癌、36例胆管癌及对照组13例胆囊胆管炎症组织中MRP1、LRP及GST-π的表达情况，采用X^2检验和Spearman检验。结果MRP1、LRP和GST-π在胆囊癌组织中的表达阳性率分别72％（13／18）、78％（14／18）和61％（11／18），在胆管癌组织中的表达阳性率为86％（31／36）、75％（27／36）和69％（25／36），显著高于对照组的23％（3／13）、23％（3／13）和23％（3／13）（X。=4．5，P〈0．05）。LRP在胆管癌年龄大于60岁组的表达阳性率93％（13／14）显著高于小于60岁组的64％（14／22）（X^2=3．9，P〈0．05）。上述指标与性别、年龄、病理分期、病理类型、分化程度和淋巴结转移均无明显相关（P〉0．05）；MRP1和GST-π在胆囊癌和胆管癌的表达具有相关性，Spearman系数=0．569（P〈0．05）。结论MRP1、LRP、GST-π在未经过化疗的胆囊癌和胆管癌组织中均有不同程度的高表达；胆囊癌和胆管癌的原发性多药耐药可能与MRP1、LRP、GST-π有关。
Objective To investigate the expression of muhidrug resistance-related protein 1 （ MRP1 ） , lung resistance-related protein （LRP） and glutathione S-transferase-π （ GST-π ） in carcinoma of the gallbladder and cholangiocarcinoma. Methods MRP1, LRP, GST-π were measured in experimental group （18 cases of carcinoma of the gallbladder, 36 cases of cholangiocarcinoma） and control group（ 13 cases of cholecystitis and cholangeitis ） by immunohistochemistry. Statistical analysis used chi-square test and spearman test. Results The positive rate of MRP1 , LRP, GST-π in carcinoma of the gallbladder and cholangiocarcinoma were 72% （ 13/18）, 78% （ 14/18）, 61% （11/18） and 86% （31/36） , 75% （27/36） , 69% （25/36） , respectively, significantly higher than those of 23% （ 3/13 ） , 23% （ 3/13 ） , 23% （ 3/13 ） （ X^2 = 4. 5, P 〈 0.05 ） in control group. The expression of LRP [ 93 % （ 13/14 ） ] in patients 〉 60 years old was significantly higher than 64% （ 14/22 ） in patients younger than 60 yrs old （ X2 = 3.9, P 〈 0. 05 ）. In addition, their expression was not related to gender, age, staging, tumor differentiation and lymph node metastasis（P 〉 0. 05 ）. The expression of MRP1 was related with those of GST-π, Spearman correlation coefficient = 0. 569 （ P 〈 0. 05 ）. Conclusions MRP1 , LRP, GST-π were over expressed in various degrees in carcinoma of the gallbladder and cholangiocarcinoma without chemotherapy, and related to the primary multidrug resistance of cholangiocarcinoma and carcinoma of the gallbladder.
出处
《中华普通外科杂志》
CSCD
北大核心
2008年第5期356-359,共4页
Chinese Journal of General Surgery
基金
上海市科学技术委员会基金资助项目（044119629）
关键词
胆囊肿瘤
胆管肿瘤
多药耐药相关蛋白质类
肺耐药蛋白
谷胱苷肽S-转移酶-π
Gallbladder neoplasms
Bile duct neoplasms
Multidrug resistance-associated proteins
Lung resistance-related protein
Glutathione S transferase-π
