摘要
目的确定2006年11月广西大新县大范围腹泻暴发性流行的病原及其分子生物学特点。方法进行流行病学个案调查和粪便标本的实验室检测，用ELISA试剂盒进行A组轮状病毒的检测，对检测阳性标本进行轮状病毒分型并对其中4份检测阳性轮状病毒的vP7全基因扩增。结果采集的64份腹泻患者粪便标本中共有30份检测为轮状病毒阳性，总阳性率为46．9％，此次暴发的流行病学和临床特征也符合轮状病毒腹泻特征，RT-PCR进行G／P分型显示为GIP[8]型轮状病毒，VP7氨基酸显示第68位氨基酸改变。结论GIP[8]型A组轮状病毒是此次腹泻暴发的病原。值得注意的是，此次A组轮状病毒暴发累及儿童和成人。
Objective To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing county, Guangxi province. Methods Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. Full-length VP7 genes of 4 positive specimens were amplified and analyzed. Results 30 cases of Rotavirus-positive were identified from 64 specimens. The attack rate was 46.9%, and G/P typing was GIP[8]. A change of VP7 amino acid residue is at positions 68. Conclusion GIP[8] rotavirus is the etiologic agents of this diarrhea outbreak. In addition, adults were included in this outbreak.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第2期104-106,共3页
Chinese Journal of Experimental and Clinical Virology
