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PCR对可疑慢性淋病病人淋病奈瑟菌的检测 被引量:2

DETECTION OF NEISSERIA GONORRHEA BY PCR IN THE SUSPICIOUS PATIENTS WITH CHRONIC GONORRHEA
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摘要 ①目的明确临床上可疑慢性淋病病人的病原学诊断。②方法用聚合酶链反应(PCR)对76例可疑慢性淋病病人的尿道分泌物进行了淋病奈瑟菌检测,并与传统的细菌分离培养法的敏感性进行了比较。③结果聚合酶链反应测出阳性标本22份(28.9%),分离培养法测出阳性标本8份(10.5%)。两种方法敏感性比较,差异有显著性(χ2=12.07,P<0.01)。④结论与细菌培养法相比PCR敏感而快速,是明确慢性淋病病原学诊断和指导治疗的好方法。 Objective To make clear the pathogenic diagnosis for the patients suspected of chronic gonorrhea. MethodsSeventysix urethral specimens taken from patients suspected of chronic gonorrhea were examined by PCR, conventional culture medium for neisseria gonorrhea were used as control. Results Twentytwo specimens were positive by PCR (28.9%), and 8 specimens were positive by conventional culture medium (10.5%). PCR was more sensitive than conventional culture medium (χ2=1207,P<001). Conclusion PCR is a sensitive and quick method for diagnosing chronic gonorrhea and helpful in guiding the treatment.
机构地区 青岛医学院
出处 《青岛医学院学报》 1997年第4期336-337,共2页 Acta Academiae Medicinae Qingdao Universitatis
关键词 淋病奈瑟菌 慢性 淋病 聚合酶链反应 neisseria gonorrhea chronic gonorrhea PCR
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