摘要
①目的探讨乙型肝炎病毒（ＨＢＶ）ＤＮＡ与增生结节和肝癌的关系。②方法应用原位杂交技术检测５８例肝细胞癌（ＨＣＣ）及癌旁组织中ＨＢＶ－ＤＮＡ的表达。③结果肝细胞癌及癌旁组织中ＨＢＶ－ＤＮＡ阳性率分别为３７．９％（２２／５８）和４４．８％（２６／５８），差异无显著性（χ２＝０．５６，Ｐ＞０．０５）。ＨＢＶ－ＤＮＡ在癌旁组织中主要位于胞浆内，而肝癌组织中以胞核定位为主。２２例ＨＢＶ－ＤＮＡ阳性的ＨＣＣ中，１６例ＨＣＣ及癌旁组织均阳性，６例ＨＣＣ单独阳性，两者差异有显著性（χ２＝９．０９，Ｐ＜０．０１）。６例癌旁增生结节中ＨＢＶ－ＤＮＡ阳性（６／２７），以胞核定位为主。④结论ＨＢＶ感染与ＨＣＣ存在密切关系。癌周组织ＨＢＶ－ＤＮＡ以复制型为主，癌组织内ＨＢＶ－ＤＮＡ以整合型为主；癌旁增生结节为ＨＣＣ的癌前病变。
Objective To study the relationship between HBVDNA and hepatocellular carcinoma (HCC),hyperplastic nodules. Methods Hybridization in situ method was used to detect HBVDNA in 58 cases of HCC and their surrounding tissues.Results The positive rate of HBVDNA in HCC and their surrounding tissues were 37.9%(22/58) and 44.8%(26/58) respectively. The difference was not signifciant(χ2=0.56,P>005). HBVDNA was mainly found in cytoplasm of pericarcinomatous tissues and in nucleus of HCC; Among the 22 HCC positive cases, 16 cases also showed pericarcinomatous tissue positive. Six cases(6/27) of hyperplastic nodules were nuclear HBVDNA positive. Conclusion HBV infection is a significant cause of HCC; HBVDNA in the pericarcinomatous tissue is of the replicative form, and in the HCC tissues, of the integrated form; hyperplastic nodule is the precancerous lesion of HCC.
出处
《青岛医学院学报》
1997年第4期313-314,共2页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省教委科技发展基金
