摘要
【目的】筛选能降解黄曲霉毒素B1(AFB1)的细菌,以期在该毒素的生物脱毒中得到应用。【方法】以香豆素为惟一碳源和能源进行AFB1降解菌株的初筛,之后将初筛的10株菌分别降解浓度为100μg·kg-1的AFB1。【结果】筛选出的NMO-3菌株降解AFB1能力达85.7%,显著高于其它菌株(P<0.01)。从形态、生理生化反应以及16SrDNA序列比对等方面分析,最终确定NMO-3菌株为嗜麦芽窄食单胞菌(Stenotrophomonas sp.)。【结论】利用香豆素作为惟一碳源和能源筛选出了黄曲霉毒素降解菌株,后期试验证明活菌制剂在2.56×1010CFU/ml剂量以下不会引起急性毒性反应,用65%硫酸铵提取的蛋白(酶)具有AFB1降解能力。
[ Objective ] The aim of this text was mainly to find the bacteria to degrade aflatoxin B 1 and realize the application of biological degradation on AFB 1. [ Method ] Using cumarin as the carbon source and energy to have the first screening, and let the ten strains which were firstly screened out to degrade aflatoxin B 1 （100 μg·kg^-1）. [ Result ] The strain NMO-3 was screened out from ten strains, the degradation ratio of AFB 1 reached 85.7%, which was obviously higher than others （P〈0.01）. With the analysis of colony morphology, physiological and biochemical experiments, and 16S rDNA gene sequence, the strain NMO-3 was finally identified as Stenotrophomonas sp. [Conclusion] Using cumarin as the carbon source and energy could screen out the AFB1 degradation strains. The microbial cells did not bring acute toxic effects when the biomass preparation rate was lower than 2.56 × 10^10CFU/ml. The multienzyme obtained by using 65% deposition ammonium sulfate could degrade AFB 1.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第5期1459-1463,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30571353)
