摘要
目的:建立一种准确测定萹蓄中总黄酮含量的新方法。方法:以芦丁为标准样品,用荧光分光度法测得其荧光强度、绘制标准曲线。用60%乙醇加热回流提取萹蓄有效成分,在相同的条件下测定样品中总黄酮的荧光强度,根据回归方程求得其含量。结果:当芦丁的浓度范围在0μg/mL～22μg/mL时,荧光强度和芦丁浓度具有良好的线性关系,线性回归方程Y=-8.973X+22.581,相关系数r=0.9771。萹蓄中总黄酮的平均含量17.05μg/mL,所得率为6.82%。结论:用荧光分光度法测定萹蓄中总黄酮的含量,操作简便、快速、准确,建议推广应用。
Objective： To establish a new method for determining total flavanoid in Polygonum aviculare. Methods： Rutin was used as standard material to determine fluorescence with spectrofluorophotometer and draw standard curve. Total flavanoid was extracted with 60 % ethanol by continuous thermal reflux. The fluorescence of it was determined in the same condition, and the content was obtained on the basis of regression equation. Results： When the rutin concentration ranged from 0 μg/mL to 22 μg/mL, fluorescence and rutin concentration showed the good linear relationship. Linearity relationship was Y=-8.973X＋22.581, and the correlative coefficient was 0.9771. The average amount of total flavanoid in Polygonum aviculare was 17.05 μg/mL, and the extracting rate of the flavones was 6.82 %. Conclusions： It is simple, rapid, and accurate method to determine total flavanoid in Polygonum aviculare with spectrofluorophotometer, and the method was suggested to be adopted.
出处
《现代生物医学进展》
CAS
2008年第7期1325-1326,共2页
Progress in Modern Biomedicine
关键词
荧光光度法
篇蓄
总黄酮
测定
Spectrofluorimetry
Polygonum aviculare
Total flavanoid
Determination
