摘要
通过ＰＣＲ扩增ＨＢｓＡｇ基因的Ｓ片段，然后将Ｓ基因与恶性疟原虫杂合４５肽基因连接并克隆到酵母表达质粒ｐＹＥＵｒａ３上。重组质粒被转化到酵母受体菌中。融合基因在酵母中获得表达，表达产物ＨＢｓＡｇ／４５肽，该融合蛋白经亲合层析后被纯化。电镜观察到ＨＢｓＡｇ／４５肽呈颗粒状。
We amplified S region fragment of HBsAg gene by PCR and connected it with the gene encoding a 45 animoacid peptide of P.falciparum . The complex gene was cloned to yeast expression vector,pYEUra3. The recombinant plasmid was identified and transformed into yeast competent cells. The fused protein HBsAg/45 mer was successfully expressed in yeast and was purified by affinity chromatography. The hybrid HBsAg/45 mer particles were observed by electron microscope. It might be shown by Dot ELISA that the 45 mer would lie on the surface of the particles.
出处
《中国寄生虫病防治杂志》
CSCD
1997年第3期161-164,共4页
Chinese Journal of Parasitic Disease Control
基金
WHO/TDR资助
