摘要
目的:改进大黄酚与大黄素的测定方法。方法:通过甲醇提取与酸水解同步进行,用高效液相色谱法简便、快捷、准确地测定了多种药材与制剂中大黄酚与大黄素含量。色谱条件为:Shimadzu VP—ODS C_(18)(150 mm×4.6 mm,5μm)为色谱柱,甲醇-0.1%磷酸(85:15)为流动相,流速:1.0 mL·min^(-1),检测波长为254 nm。结果:通过方法学考察,大黄酚的进样量在0.022～0.32μg(r=0.9999),大黄素的进样量在0.015～0.22μg(r=0.9999)范围内,呈良好的线性关系。大黄酚与大黄素的平均回收率(n=3)分别为96.10%～101.7%和96.75%～100.9%。结论:改进方法检测效率高,检测成本低,环境污染轻。
Objective：To improve a method for determination of chrysophanol and emodin. Method：The contents chrysophanol and emodin in several herbals and preparations were determinated simply, rapidly and accurately by HPLC（after extraction by methanol and decomposed with dilute acid）. The chromatography was performed by C18 column （150mm×4.6mm,5μm）with MeOH -0. 1% H3PO4 （85：15 ）as the mobile phase and absorbance was monitored at 254 mm. The flow rate was 1.0 mL·min^-1. Results：The methodological study showed that a good linear correlation existed in the range of 0.022 - 0.32μg （r= 0.9999 ） of chrysophanol, and 0.015 - 0.22μg （r= 0.9999）of emodin respectively. The average recovery of chrysophanol was 96. 10% -101.7% （n =3）and that of emodin was 96.75 % - 100. 9% （n = 3 ）. Conclusion：This improved method is high efficient of determination with low cost and light environmental pollution.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第3期461-465,共5页
Chinese Journal of Pharmaceutical Analysis
