摘要
目的探索H2O2诱导大鼠海马细胞氧化损伤模型的最佳浓度。方法应用不同浓度的H2O2与大鼠海马细胞共同培养24 h。观察细胞形态学变化;MTT法测定细胞活性;免疫组化法检测细胞中Bax与Bcl-2的蛋白表达。结果与正常组细胞比较,当H2O2浓度高于1 mmol/L时细胞形态损伤明显加重,细胞活性降低,bax蛋白高度表达;当H2O2浓度低于1 mmol/L时细胞形态无明显损伤,细胞活性无明显改变,bcl-2蛋白高度表达。结论在本实验条件下H2O2浓度1 mmol/L时为模型最佳复制浓度。
Objective To explore oxidative damage in rats. Method the best concentration of H2O2 to induce hippocampal cellular The hippocampal cell from the rat was co-cultured with H2O2 in diversity concentration for 24 hours. The changes of the cell morphology was observed, the viability of hippocampal neures was detected with MTT method; the expression of Bax and Bcl-2 were detected by immunohistochemical method. Result Compared with normal group, when the concentration of H2O2 is higher than 1mmol/L,the morphology damage of hippocampal cell is obviously aggravated, the eytoactive is low,the Bax is high expression. When the concentration of H2O2 is lower than 1mmol/L, the morphology damage of hippocampal cellular is not obviously aggravated, the cytoactive has no obvious change, the Bcl-2 is high expression. Conclusion The best concentration of H2O2 to establish cell oxidative damage model is 1mmol/L.
出处
《实验动物与比较医学》
CAS
2008年第2期121-124,128,共5页
Laboratory Animal and Comparative Medicine
基金
湖北省自然科学基金(2001ABB174)
